N^+注入诱变芽孢杆菌选育高产抗菌物质菌株  

THE SCREENING OF Bacillus subtilis STRAIN WITH HIGH-PRODUCED ANTIMICROBIAL SUBSTANCE USING N^+ ION IMPLANTATION

作　　者：沈娟[1] 别小妹[1] 陆兆新[1] 吕凤霞[1] 朱筱玉[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《核农学报》2006年第4期296-298,330,共4页Journal of Nuclear Agricultural Sciences

基　　金：江苏省高新技术资助项目(BG2003311)

摘　　要：本研究通过N+注入芽孢杆菌选育高产抗菌物质菌株。注入N+的能量为25keV,剂量为50×2.6×1013、80×2.6×1013、100×2.6×1013、120×2.6×1013和150×2.6×1013N+/m2。结果表明最佳的注入剂量为50×2.6×1013N+/m2,选育出了1株高产菌株(Bacillus subtilisfmbJ224),其抗菌物质产量是出发菌株的1.96倍,经传代培养,其高产性能稳定。同时通过对该菌株的发酵特性研究,发现其产抗菌物质的模式为延滞合成型。N^＋ ion implantation was used to obtain higher-yield antimicrobial substance. Bacillus subtilis fmbJ was mutated by 25keV N^＋ ion implantation with the dose of 50 × 2.6 × 10^13, 80 ×2.6 × 10^13, 100 × 2.6 × 10^13, 120 × 2.6 × 10^13 and 150 × 2.6 × 10^13 N^＋/m^2. Results showed that the optimal N^＋ ion dose was 50 ×2.6 × 10^13 N^＋/m^2, and a strain of high-yield antimicrobials was obtained and named as Bacillus subtilis fmbJ224. Its antimicrobial substance yield was increased by 96 % than the initial. The fermentation characteristic of the strain was studied, and the mode of producing antimicrobial substance for the selected strain was arrearage synthesis type.

关 键 词：N^+离子 芽孢杆菌 抗菌物质 突变体 

分 类 号：TS202.3[轻工技术与工程—食品科学]