降解纤维素菌株的筛选  

Study on screening of cellulose degradation strains

作　　者：伍时华[1] 徐雅飞[1] 黄翠姬[1] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006

出　　处：《食品科技》2006年第8期50-52,共3页Food Science and Technology

基　　金：广西科学基金项目(桂科自0481027)

摘　　要：通过羧甲基纤维素琼脂平板、滤纸条培养基从自然环境中筛选到4株高效纤维素降解菌,其中木霉2株,青霉2株,分别记为T-1、T-2和P-1、P-2。他们都能利用羧甲基纤维素,在羧甲基纤维素平板上长势良好,而且能使滤纸条较快的崩溃。然后将T-1、T-2和P-1、P-2以及一株康氏木霉13022分别接种至以甘蔗渣为主要碳源的发酵培养基上,于28℃恒温培养,从84h开始每隔24h分别测定各相应发酵产物的纤维素酶活。结果显示T-1、T-2和P-1、P-2具有较高的产纤维素酶能力,其中T-1具有较高的羧甲基纤维素酶活力和滤纸酶活力,而P-1、P-2具有较高的产β-葡萄糖苷酶的能力。4 species of fungi including 2 species of Tdchoderma T-1, T-2 and 2 species of Penicillium P-1, P- 2 were selected from nature environment via carboxymethyl cellulose agar plate and filter paper medium. T-1, T-2, P-1, P-2 and a species of Trichoderma koningii13022 were inoculated to the fermentation medium which use the sugarcane bagasse as the main carbon source after 84 hours of culture at the temperature of 28℃, the cellulase activity of every fermented products were determined. It was found that T-1, T-2, P-1, P-2 own high cellulase productivity, but T-1 have higher carboxymethyl-cellulase activity and filter paper enzyme activity, and P-1 ,P-2 has higher （3-glucosaccharase activity.

关 键 词：筛选 纤维素酶 滤纸酶 羧甲基纤维素酶 Β-葡萄糖苷酶 

分 类 号：TS201.3[轻工技术与工程—食品科学]