水解淀粉的酿酒酵母菌的构建  

THE CONSTRUCTION OF STARCH DIGESTIBLE STRAIN OF SACCHAROMYCES CEREVISIAE

作　　者：唐国敏[1] 郗乔然[1] 钟丽婵[1] 杨开宇[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》1996年第4期250-255,共6页Acta Microbiologica Sinica

基　　金：国家八五攻关项目

摘　　要：把黑曲霉糖化酶cDNA,酵母磷酸甘油激酶基因启动子区和终止区以及酵母Ty因子的δ序列构建成整合型的糖化酶表达分泌质粒pKG 1。该质粒转化酿酒酵母Y33得到整合型转化子。转化子分泌糖化酶活力在3.0μ/ml以上,在以5％可溶性淀粉为碳源的培养基中静止培养7d,淀粉利用率达86％,生成酒精的浓度与以5％葡萄糖为碳源时相等。An integration type plasmid pKG1 for expression and secretion of glucoamylase was constructed by insertion of glucoamylase cDNA from Aspergillus niger between yeast phosphoglycerate kinase (PGK) promoter and terminator regions. The plasmid contained the sequence of yeast Ty element, which was used as a homologous fragment for intergration. The activity of glucoamylase secreted by yeast strain transformed with pKGl was over 3.0 u / ml. The test of alcohol fermentation with the transformants were conducted in the medium containing 5% of the soluble starch as the sole carbon source at 30℃ for 7 days. The results showed that the transformants utilized over 86% Of the starch and produced the same concentration of alcohol as that in the medium containing 5% of glucose as the carbon source.

关 键 词：水解淀粉 酿酒酵母 淀粉利用 酒精 发酵 

分 类 号：TS261.11[轻工技术与工程—发酵工程]