重组海藻糖合成酶工程菌的pH-stat高密度发酵工艺研究  

Expressing of trehalose synthase by ph-star high cell-density fermentation

作　　者：蒙健宗[1] 陈发忠 王青艳[1] 黄日波[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530004 [2]南宁中诺生物工程有限责任公司,广西南宁530003

出　　处：《食品工业科技》2006年第8期125-128,共4页Science and Technology of Food Industry

基　　金：国家"863"计划(AA214171)

摘　　要：重组海藻糖合成酶工程菌的分批补料发酵中,用氨水调节培养液的pH为6.78～6.80,初糖耗尽后按培养液的pH从6.80起每升高0.01即开始补充葡萄糖至0.7g·L-1。据此补料策略可将发酵过程的乙酸积累控制在6.26g·L-1以下;补料发酵12h后,即获得细胞干重为51.5g·L-1的细胞高密度,细胞密度是分批培养的7.5倍;诱导后重组海藻糖合成酶表达率为15.2%,单位体积内的表达量是分批培养的4.5倍。pH-stat fed-batch fermentation of recombinant Escherichia coli was carried out in 40L biostat fermentator. Feeding solution was added when the pH rose to a value greater than its set point by 0.01. After 12h feeding, the dry cell weight reached as high as 51.5g·L^-1 and the acetic acid was only 6.26g·L^-1 When ceils were induced by IPTG, the expression of trehalose synthase was accounted for 15.2% of total soluble proteins.

关 键 词：海藻糖合成酶 高密度发酵 PH-STAT 

分 类 号：TS201.2[轻工技术与工程—食品科学]