双酶法降解玉米麸皮制备低聚糖和阿魏酸的研究  

Release of Ferulic Acid and Xylo-Oligosaccharides from Maize Bran

作　　者：薛枫[1] 欧仕益[1] 汪勇[1] 黄才欢[1] 

机构地区：[1]暨南大学食品科学与工程系,广东广州510632

出　　处：《粮食与饲料工业》2006年第10期17-19,26,共4页Cereal & Feed Industry

基　　金：广州市科技攻关重点项目;课题编号:2003Z3-E0061

摘　　要：利用阿魏酸酯酶和阿拉伯木聚糖酶的混合酶制剂可以实现高效降解玉米麸皮,制备功能性食品原料阿魏酸和低聚木糖。探讨了利用双酶法降解去淀粉玉米麸皮的工艺条件。确定了酶的最适反应条件:温度40℃、pH值4.4,料液比8%。在最适条件下酶解24 h,可以释放出去淀粉玉米麸皮中23.5%的阿魏酸和18.4%的还原糖。探讨了高温高压预处理底物对酶解的影响,结果表明在160℃(0.52 MPa)的高温下预处理去淀粉玉米麸皮30 m in,酶解24 h后释放阿魏酸的量比未经高温高压预处理时提高1倍,还原糖量提高3倍。A high-effective degradation of maize bran can be realized by a mixture of ferulic acid esterase and arabinoxylanase for preparation of raw materials for functional foods ferulic acid and xylo-oligosaccharides. The technological conditions for degradation of destarched maize bran by a double-enzymatic method were discussed. The optimal conditions of enzymatic reaction were determined： temperature 40℃, pH 4.4 and ratio of feed to liquid 8%. Under these conditions, 23.5% of ferulic acid and 18.4% of reducing sugar were released by enzymatic hydrolysis for 24 hours. Furthermore, the effects of pretreatment of the substrates under high temperature and high pressure on the enzymatic hydrolysis were discussed. The results showed that when pre-treating the destarched maize bran under high temperature （ 160℃） and high pressure （0.52 MPa） for 30 min, the released ferulic acid and reducing sugars were doubled and tripled respectively after enzymatic hydrolysis for 24 hours in comparison with that without pretreatment under high temperature and high pressure.

关 键 词：玉米麸皮 阿魏酸酯酶 阿拉伯木聚糖酶 高温高压 

分 类 号：TQ914.1[化学工程]