浒苔多糖的分离纯化及其对非特异性免疫功能的体外实验研究  

Studies on Separation and Purification of Polysaccharides in Enteromorpha and Its Effects on Non-specific Immunity in Vitro

作　　者：徐大伦[1] 黄晓春[1] 杨文鸽[1] 薛长湖[2] 王海洪[1] 

机构地区：[1]宁波大学生命科学与生物工程学院,宁波315211 [2]中国海洋大学食品科学与工程系,青岛266003

出　　处：《中国食品学报》2006年第5期17-21,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：浙江省教育厅项目(No.20051708)

摘　　要：用离子交换层析、凝胶层析等方法对浒苔多糖进行了分离纯化,得到2个组分EP1和EP2。对EP2进行了非特异性免疫功能的体外实验研究,结果表明:适宜浓度的EP2能明显促进T、B淋巴细胞的增殖反应,而且对抗原提呈细胞活化所致的诱导IFN-g的产生有非常显著的增强作用,但对ConA诱导T细胞直接活化所致的IFN-g和IL-2的产生未见显著的影响,说明EP2有增强免疫反应的作用。Polysaccharides in Enteromorpha were separated and purified by ionic chromatography and gel exclusion chromatography, and two fractions were obtained： EP1 and EP2. The effects of EP2 on non-specific immunity were studied in vitro. The results indicated that the proper concentration EP2 could promote the ability of T lymphocytes and B lymphocytes in evidence. Also in proper concentration EP2 showed evident effection on the production of IFN-γ and IL- 2 induced directly by T lymphocytes, however, it showed in significant effection on the production of IFN-γ induced by antigen presenting cells. So, EP2 had enhanced immune activity.

关 键 词：浒苔 多糖 分离 免疫细胞 

分 类 号：TS201[轻工技术与工程—食品科学]