不同树莓品种超氧化物歧化酶分离纯化及酶活力试验研究  

Separation,Purification and Activity determinatiom of Superoxide Dismutase from the different Raspberry

作　　者：宣景宏[1] 孟宪军[1] 刘春菊[2] 张春艳[1] 

机构地区：[1]沈阳农业大学食品学院 [2]江苏省农业科学院,南京210000

出　　处：《北方果树》2006年第6期11-13,共3页Northern Fruits

摘　　要：作者对树莓SOD粗酶液经硫酸铵分级沉淀、SephadexG-100柱层析,同时对比DEAE-52柱层析和金属螯合亲和柱层析,并对其部分性质进行研究。从树莓中纯化得到三种Mn-SOD同工酶:SODⅠ、SODⅡ、SODⅢ。实验分析结果表明,经金属螯合亲和柱层析的回收率和纯化倍数远高于DEAE-52柱层析。对5个树莓品种的酶活力研究表明熏早红的酶活力最高,美22号的酶活力最低。SOD Ⅰ ,SOD Ⅱ and SOD m were purified from the raspberries by ammonium-sulfate,gel filtration on SephadexG-100 and comparing DEAE-52 on exchange chromatography with Metals Chelating Affinity Chromatogram.And some of its characters were analyzed.The results showed that the specific activity and the recovery of the three enzymes from Metals Chelating Affinity Chromatogram were superior to that from DEAE-52 on exchange chromatography.The conclusoins on difference of SOD activity determinatiom of the different raspberry, Early Red has the most Activity determinatiom of SOD and A-22 has the least one.

关 键 词：树莓 超氧化物歧化酶 分离纯化 酶活力 

分 类 号：S663.2[农业科学—果树学] Q55[农业科学—园艺学]