几种效应物对黑曲霉果胶酶活力的影响  

Effects of Several Effectors on the Activity of Pectinase from Aspergillus niger

作　　者：林建城[1] 王志鹏[1] 杨文杰[1] 朱丽华[1] 

机构地区：[1]莆田学院环境与生命科学系,福建莆田351100

出　　处：《云南师范大学学报（自然科学版）》2006年第6期48-51,共4页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：福建省教育厅科技项目(JA05320);莆田市科技计划项目(2005N12)资助课题

摘　　要：研究了几种效应物对黑曲霉果胶酶活力的影响。结果表明:变性剂脲与SDS对果胶酶活力的抑制作用呈现出浓度效应,0.5 mmol/LSDS能使酶活力下降95.9%,5 mol/L的脲能使果胶酶活力下降95.6%;三种伯醇和丙酮对果胶酶活力具有抑制作用,15%的甲醇、乙醇、正丙醇和丙酮可分别使果胶酶活力下降18.8%、25.5%、36.1%和84.4%;而乙二醇和丙三醇对酶活力的影响表现为激活效应,15%的乙二醇和丙三醇可分别使酶活性提高22.9%和6.9%;Cu2+和Fe3+对果胶酶活力的抑制作用呈浓度梯度效应,EDTA对酶活力没有影响,说明果胶酶为非金属酶类。The effects of several effectors on the activity of pectinase from Aspergillus niger were studied. The inhibitory effects of urea and sodium dodecyl sulfate（SDS）on the enzyme were concentration dependent. 0.5 mmol/L of SDS led enzyme activity to lose by 95. 90% while 5 mol/L of urea led enzyme activity to lose by 95. 60%. When the concentrations of methanol, alcohol, propanol and acetone reached 15%（volume fraction）, the enzyme activity dropped by 18. 80%,25.50%,36. 1% and 84. 40%, respectively. However, the enzyme was activated in glycol and glycerol, when concentrations of glycerol and glycol reached 15%, the enzyme activity increased by 22.90% and 6. 90%, respectively. Meanwhile, the inhibitory effects of Cu^2＋ and Fe^3＋ on the enzyme were concentration dependent. Ethylenediaminetetraacetic acid （EDTA） had no effect on the enzyme. It indicated the pectinase wasn？ t metallic enzyme.

关 键 词：果胶酶 效应物 酶活力 

分 类 号：Q356.1[生物学—遗传学]