广东汉族人群21号染色体4个短串联重复序列多态性  

Polymorphisms analysis of short tandem repeat loci D21S1433,D21S1442,D21S1444,D21S2051 in Guangdong Han nationality in China

作　　者：杨昕[1] 廖灿[1] 黄以宁[1] 李焱[1] 易翠兴[1] 吴韶清[1] 潘敏[1] 胡顺妍[1] 袁思敏[1] 

机构地区：[1]广州市妇婴医院优生围产保健中心,510180

出　　处：《中华医学遗传学杂志》2006年第6期689-691,共3页Chinese Journal of Medical Genetics

基　　金：广州市科技局重点科研项目(2004E1-E0011)~~

摘　　要：目的调查广东地区汉族人群4个短串联重复序列D21S1433、D21S1442、D21S1444、D21S2051的多态性分布。方法采用荧光定量PCR(quantitativefluorescencePCR,QF-PCR)技术,对广东地区200份无亲缘关系样本进行等位基因分型,计算4个短串联重复序列的基因频率、基因型频率,等位基因杂合度,期望杂合性、多态信息含量和非父排除率。结果D21S1433、D21S1442、D21S1444、D21S2051分别检出9、10、9、5个等位基因,等位基因型分布均符合Hardy-Weinberg平衡(P>0.05)。D21S1433和D21S1442的杂合度较高,分别为0·818和0·820,D21S2051的杂合度最低,为0.261。结论D21S1433、D21S1442、D21S1444具有较高的杂合度和多态信息量,作为遗传标记较为理想;D21S2051杂合度较低,不能作为遗传标记物。Objective To investigate the polymorphic distribution of short tandem repeat（STR） sequences D21S1433, D21S1442, D21S1444, D21S2051 in Guangdong Han nationality in China. Methods Using quantitative fluorescens PCR technology, the authors analyzed 200 unrelated samples to acknowledge the allele frequency, heterozygesity and other genetic information. Results D21S1433, D21S1442, D21S1444, D21S2051 were tested in 200 sampies, which were tested to be statistical according to Hardy-Weinberg equilibrium （P 〉 0.05）, 9, 10, 9 and 5 alleles were detected separately in each STRs. The heterozygesity of each STR was 0.818, 0.820, 0.770, and 0.261. The polymorphic information content 〉 0.7 in D21S1433, D21S1442, D21S1444, while D21S2051 owned only 0.247 polymorphic information. Conclusion D21S1433, D21S1442, D21S1444 are found to have high heterozygesity and polymorphic information content, and they could provide useful markers for genetic purposes, while D21S2051 is not informative in Guangdong Han nationality in China.

关 键 词：短串联重复序列 遗传多态性 等位基因 

分 类 号：R394[医药卫生—医学遗传学]