冷藏养殖大黄鱼细菌相组成和优势腐败菌鉴定  

Bacterial flora and identification of dominated spoilage organisms on cultured Pseudosciaena crocea at chilled storage

作　　者：郭全友[1] 杨宪时[1] 许钟[1] 吴建军 周彩华[3] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090 [2]上海德记行科技发展有限公司,上海200086 [3]上海水产大学食品学院,上海200090

出　　处：《水产学报》2006年第6期824-830,共7页Journal of Fisheries of China

基　　金：农业部948项目(2001-478)

摘　　要：从菌落和细胞形态、生理生化特征、细胞脂肪酸组成及同源性分析等方面，结合使用Sensititre、BioFosun、MIDI细菌鉴定系统对0℃、5℃冷藏养殖大黄鱼货架期终点细菌相进行定性和定量研究，确定优势腐败菌。0℃、5℃冷藏优势菌为腐败希瓦氏菌，比例分别为75．5％、59．6％，在营养琼脂和胰酶解大豆胨琼脂培养基上菌落圆状隆起，边缘整齐，透明，无色至粉红色，革兰氏阴性杆菌，极生单鞭毛，大小为（0．6-0．9）μm×（1．5-3．3）μm，最适生长温度25-35℃、最适pH7-9。菌株氧化酶和H2S呈阳性，能还原TMAO、液化明胶和Tween40，利用N-乙酰基-D-葡萄糖胺等有机物。菌体主要脂肪酸为16：1ω7c、iso-15：0、17：1ω8c、16：0，比例分别为21．31％、13．63％、9．82％、9．76％。菌株测试特征与细菌鉴定系统数据库比照，确定优势腐败菌为腐败希瓦氏菌。Bacterial flora and identification of dominated spoilage organisms on cultured Pseudosciaena crocea at the end of shelf life during storage at 0 ℃ and 5 ℃ were qualitatively and quantitatively investigated, based on the colony and cellular morphology, biochemical and physiological characteristics as well as whole-cell fatty acid composition, and applied with Sensititre,BioFosun,MIDI bacteria identification systems. The dominated bacteria of Shewanella putrefaciens were 75.5% and 59.6% at the end of shelf life at 0 ℃ and 5 ℃, and its colonies on nutrition agar and trypticase soy broth agar were global apophysis, trim side, smooth clarity, achromatism to pink. The cells were gram negative rode （0.6 - 0.9）μm× （ 1.5 - 3.3）μm] and motile by means of polar flagellum, the optimum growth occurred at 25 - 35 ℃ and pH 7 - 9. Swains were positive for oxidation, H2S, TMAO, gelatin and Tween 40. SWains utilized N- acetyl- D- glucosamine, ect. The dominant fatty acids were 16： 1ω8c, iso- 15 ： 0,17： 1ω8c, 16： 0, mean contents were 21.31% ,13.63%,9.82%,9.76%, respectively. Results from tested swains were compared with database values in the bacterial identification systems, indicating the dominated spoilage organisms were Shewanella putrefaciens.

关 键 词：大黄鱼 细菌相 优势腐败菌 腐败希瓦氏菌 

分 类 号：S984.11[农业科学—捕捞与储运]