固相萃取-高效液相色谱法测定鸡肉中氯霉素的残留  

Determination of Chloramphenicol Residues in Chichen by SPE-HPLC

作　　者：何方奕[1] 李铁纯[1] 李学程[1] 许可[1] 回瑞华[1] 

机构地区：[1]鞍山师范学院化学系,辽宁鞍山114005

出　　处：《食品科学》2006年第12期629-630,共2页Food Science

摘　　要：本文采用固相萃取-高效液相色谱法对肉食鸡中氯霉素残留进行检测。样品用乙酸乙酯提取,并用正己烷脱酯,经ENVI-18固相萃取小柱净化后,采用C18色谱柱分离,流动相甲醇:水=35:65(V/V),流速为1ml/min液相色谱分析,紫外检测波长为280nm。在1～200μg/ml范围内,峰面积与氯霉素浓度呈良好的线性关系(r=0.9992),精密度(RSD)小于5%,检测限为0.005μg/g,平均回收率为82.4%。The chioramphenicol in chichen was assayed with the method of solid phase extraction and high performance liquid chromatography （HPLC）. The chichen sample was extracted by ethylacetate and cleaned by ENVI- 18 SPE column. Then the sample was separated by the C18 column with a mobile phase of methanol and water（35：65, V/V）, at the rate of 1ml/min and detected at 278nm. The RSD of this method is less than 5%, and the detection limit was 0.005μg/g.The everage recoveries was 82.4%.

关 键 词：氯霉素(CAP)残留 鸡肉 固相萃取(SPE) 高效液相色谱(HPLC) 

分 类 号：TS207.5[轻工技术与工程—食品科学]