壳聚糖固定化超氧化物歧化酶的研究  

Study on Supeoxide Dismutase Immobilized with Chitosan

作　　者：张春艳[1] 孟宪军[1] 宣景宏[1] 

机构地区：[1]沈阳农业大学食品学院,辽宁沈阳110161

出　　处：《食品科学》2007年第3期212-215,共4页Food Science

摘　　要：目的:研究壳聚糖固定化超氧化物歧化酶的酶学性质。方法:分别以不同方法对超氧化物歧化酶进行固定并比较其活力,对固定化方法进行相应的优化,对固定化超氧化物歧化酶进行酶学性质测定。结果:以壳聚糖为载体,戊二醛交联法制备固定化超氧化物歧化酶,优化条件下制备的固定化酶,所得固定化酶活力为330U/g,酶活回收率为58.33%,热稳定性和酸稳定性较游离酶有很大的提高,且具有良好的贮存稳定性,固定化酶可实现反复使用,提高了利用率。结论:壳聚糖-戊二醛交联法可用于制备性能较优的固定化超氧化物歧化酶。Objective： To study the enzyme properties of superoxide dismutase immobilized with chitosan. Methods： Immobilized superoxide dismutase were prepared by different processes. The methods were optimized by comparing their activities. Results： Superoxide dismutase on chitosan with glutaraldehyde as erosslinking agent showed the highest activities and recovery rates.The activity of superoxide dismutase powder immobilized under optimum conditions is 330 U/g,with the recovery rate above 58.33%.The temperature stability and the pH stability of the immobilized enzyme are much better than the free enzyme.And the immobilized enzyme also shows a good storage stability. The immobilized enzyme may be applied repeatedly. It can improve untilization rate. Conclusion： The immobilized superoxide dismutase can be prepared by crosslinking glutaraldehyde on chitosan, and it possesses good properties.

关 键 词：超氧化物歧化酶 壳聚糖 固定化酶 

分 类 号：Q814[生物学—生物工程]