蛋白芯片法快速测定食品中氯霉素和磺胺二甲嘧啶残留  

CAP and SM_2 Residues Quantitation and Fast Detection in Foodstuff by Protein Microarray

作　　者：左鹏[1] 叶邦策[1] 

机构地区：[1]华东理工大学食品科学与工程系生物反应器工程国家重点实验室,上海200237

出　　处：《食品科学》2007年第2期254-258,共5页Food Science

基　　金：国家863计划重大专项食品安全项目(2002AA2Z2007-1);上海市重点攻关项目(044319221)

摘　　要：人们对食品中药物残留检测方法提出了快速检测、准确定量的要求。本文研究了蛋白芯片法快速定量检测食品中氯霉素和磺胺二甲嘧啶残留。芯片上固定氯霉素和磺胺二甲嘧啶人工抗原,芯片反应区内加入氯霉素和磺胺二甲嘧啶单克隆抗体和其标准品或待测样品混合物,待抗原抗体完全反应后加入荧光标记二抗示踪。不同浓度的竞争标准品与检测荧光强度作标准曲线,然后通过标准曲线对待检试样中氯霉素和磺胺二甲嘧啶残留量进行定量分析,并同现行的免疫学测定方法进行了比较。This study was carded about the veterinary drug residue quantitation and fast detection in foodstuff by protein microarray. The Chloramphenicol（CAP） and Sulfamethazine（SM2） synthetic-antigen coupled with the carder protein BSA（bovine serum albumin） were spotted on the microarray. After immobilization, the antibody for these two residues and the standard or sample mixture were added.Then the Cy5 labeled secondary antibody was added after antigen-antibody reaction. The calibration curve of these two residues was established to indicate the lowest detection limit and the area the linearity range.With the calibration curve,we can calculate the concentration of the unknown sample.

关 键 词：蛋白芯片 兽药残留 氯霉素 磺胺二甲嘧啶 

分 类 号：R155.5[医药卫生—营养与食品卫生学]