绿色木霉(Trichoderma viride)_(867)产壳聚糖酶的发酵工艺条件的优化  

Culture medium optimization for producing chitosanase by Trichoderma viride

作　　者：段文凯[1] 郑春翠[1] 周晓云[1] 吕美巧[1] 江蕾[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,浙江杭州310032

出　　处：《浙江工业大学学报》2007年第1期41-45,共5页Journal of Zhejiang University of Technology

基　　金：浙江省科技厅重点资助项目(2004C21005)

摘　　要：系统研究了碳源、氮源、初始pH、培养温度、培养基装液量、接种量和培养时间等因素对绿色木霉867产壳聚糖酶的影响.结果表明,最佳碳、氮源分别为可溶性壳聚糖和蛋白胨,在初始pH5.0,培养温度28℃,培养基装量75 mL/250 mL,接种量6%和培养时间(180 r/min)40 h时最利于产酶.在此基础上通过均匀设计法优化了发酵培养基配方.优化后的培养基配方为:可溶性壳聚糖0.9%,氨基葡萄糖0.5%,蛋白胨0.9%,K2HPO40.016%,CaCl2.2H2O 0.055%.在该条件下,壳聚糖酶活为0.291 u/mL,比原基础培养条件下酶活提高29.9%.The effect of a number of factors on the production of chitosanase was studied, including the carbon source, nitrogen source, pH value, culture temperature, volume of culture media, innoculation and culture time. The results showed that the optimal carbon source and nitrogen source were soluble chitosan and peptone, respectively. The production of chitosanase was optimal with an initial pH value of 5. 0, the culture temperature of 28 ℃, and Trichoderma viride inoculated with 6% inoculum on 180 r/min in 250 mL with 75 mL medium for 40 h. The composition of fermentation medium was optimized through uniform-design. A suitable fermentation condition was found as follows： soluble chitosan 0.8 %, glucosamine 0.5 %, peptone 0.9 %, K2HPO4 0. 016%, CaC12 · 2H2O 0. 055%. Chitosanase activity reached 0. 291 u/mL under this condition, 29.9% higher than that under the basic fermentation condition used previously.

关 键 词：壳聚糖酶 绿色木霉 发酵 工艺条件优化 均匀设计 

分 类 号：Q935[生物学—微生物学]