猪肌肉组织LPL基因表达的发育性变化及其与肌内脂肪沉积关系的研究  

The Developmental Changes of LPL mRNA Expression in Muscle and Their Association with Intramuscular Fat for Pigs

作　　者：王刚[1] 曾勇庆[1] 武英[2] 魏述东[3] 包新见[1] 刘婵娟[1] 孙延晓[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省农科院畜牧兽医研究所,济南250100 [3]山东省莱芜市畜牧兽医技术推广中心,莱芜271100

出　　处：《畜牧兽医学报》2007年第3期253-257,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家畜禽品种资源重点保护项目(200014);山东省农业良种工程重大课题优质瘦肉猪配套系选育项目(20059913);山东农业大学青年科技创新基金项目

摘　　要：以40-90 kg 6个体重组的莱芜猪和鲁莱黑猪共72头去势公猪为试验对象(每组6头),采用相对定量RT-PCR方法,以-βactin基因为内标,分析肌肉组织LPL基因表达的发育性变化及其与肌内脂肪沉积的关系。结果表明:莱芜猪与鲁莱黑猪肌肉组织中LPL基因表达的发育性变化趋势基本相似,随体重的增长,莱芜猪50-70kg阶段LPLmRNA表达丰度逐渐下降(P<0.05),80 kg阶段迅速回升出现一个峰值后再次下降;鲁莱黑猪LPLmRNA表达丰度随体重的增加呈缓慢下降趋势,70 kg以后变化不大并维持较低水平,其前期(40-60 kg)与后期(70-90 kg)的表达量差异显著(P<0.05)。总体上莱芜猪肌肉组织LPL基因表达量略高于鲁莱黑猪(P>0.05)。相关分析表明,莱芜猪和鲁莱黑猪肌内脂肪相对含量与肌肉组织LPLmRNA表达的发育性变化分别呈显著(P<0.05)和极显著(P<0.01)的正相关。研究结果提示:猪肌肉组织LPL是肌内脂肪沉积的重要参与者,其编码基因的表达具有明显的体重发育特征,并对肌内脂肪的沉积具有一定程度影响。To investigate the developmental changes of lipoprotein lipase （LPL） mRNA expression in pig muscle and their association with intramuscular fat （IMF）, relative quantitative RTPCR with β-actin mRNA as internal standard was used in this study. Seventy-two Laiwu Black （LW） and Lulai Black （LL） castrated boars were fed in groups and slaughtered at weight of 40- 90 kg （n=6）. Results indicated that the developmental tendencies of muscle LPL mRNA in LW and LL pigs were similar. With gaining of body weight, the LPL mRNA abundance decreased from 50 kg to 70 kg （P〈0.05）, but increased and presented a peak at 80 kg phase for LW. The LPL mRNA abundance decreased gently, and significant difference was found between 40-60 kg and 70-90 kg phases for LL. On the whole, the expression level of LPL mRNA in muscle of LW tended to he higher than that of LL （P〉0.05）. Correlation analysis showed that the expression of LPL mRNA in muscle were positively correlated with the ratio of IMF to hodyfat for both LW （P〈0.05） and LL pigs （P〈0.01）. The results suggest that LPL in muscle is one of the most important actors involved in IMF deposition, also the LPL gene expression is affected by body weight development, and has a certain extent effect on IMF content .

关 键 词：莱芜猪 肌内脂肪 LPL mRNA 发育性变化 RT-PCR 

分 类 号：S828.2[农业科学—畜牧学]