两歧双歧杆菌耐氧耐酸耐胆盐优良菌株的选育  

The breeding of novel oxygen-、acid-and bile salt-resistant Bifidobacterium bifidum

作　　者：熊三玉[1] 管斌[1] 董书阁[1] 王艳[1] 郑君[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国食品添加剂》2007年第2期126-130,共5页China Food Additives

摘　　要：本实验通过反复驯化的培养方法将两歧双歧杆菌驯化成耐氧耐酸耐胆盐的优良菌株。在耐氧驯化时,通过增加培养基中的氧气分压的方法进行驯化;在耐酸驯化的过程中,通过降低培养基的初始pH值的方法进行。实验结果表明,在牛乳培养基中耐氧驯化后的双歧杆菌的增殖比例为42000%,而原始菌的增殖比例仅为8333%,且前者的凝乳时间较后者短,为后者的2/3;再经过耐酸驯化的菌株在低pH值条件下的产酸性能也大为提高,且耐酸性明显优于未经驯化的菌株;最后再经过胆盐驯化的菌株在胆盐浓度为0.5%时培养后也有较高的活菌数,说明其对胆盐的忍耐力明显增强。The Bifidobacterium bifidum were domesticated with the traditional cultivation method. In the oxygen-resistance domestication, we increase the oxygen pressure to domensticate the Bifidobacterium bifutum. And in the acid-resistance domestication, the Bifidobacterium bifidum were domesticated by lowering the pH of the liquid medium. The results show that the capability of the domesticated Bifidobacterium bifidum is superior to the original strains. In the milk medium, the growth rate for the domesticated strains is 42000%, and the original strains is only 8333%. The production of the acid is more than the original strains, and the milk-coagulating time has been shortened to 2/3. In the bile saltresistance domestication, while the bile salts concentration is 0. 5%, the concentration of the domesticated one can also reach to as high as 1×10^6.

关 键 词：两歧双歧杆菌 耐氧 耐酸 耐胆盐 驯化 

分 类 号：TS201.3[轻工技术与工程—食品科学]