复合酶水解玉米皮制备阿魏酸和低聚糖的研究  

PREPARATION OF FERULIC ACID AND OLIGOSACCHARIDE FROM MAIZE BRAN BY FERULIC ACID ESTERASE IN COMBINATION WITH ARABINOXYLANAES,CELLULASE AND VISCOZYME L

作　　者：朱易佳[1] 欧仕益[1] 

机构地区：[1]暨南大学食品科学与工程系,广东广州510632

出　　处：《食品研究与开发》2007年第5期68-71,共4页Food Research and Development

基　　金：广州市科技攻关重点项目(2003Z3-E0061)

摘　　要：研究采用阿魏酸酯酶、阿拉伯木聚糖酶、纤维素酶和ViscozymeL(戊聚糖复合酶)共同作用于去淀粉玉米皮,制备阿魏酸和低聚糖。结果表明,当混合酶制剂(阿魏酸酯酶和阿拉伯木聚糖酶)的添加量与底物干重比为6∶1、纤维素酶和ViscozymeL的添加量均为底物干重的5%时,在pH5.5,45℃下酶解24h,能得到最高的阿魏酸和低聚糖产量;在此条件下能释放出去淀粉玉米皮中25.4%的阿魏酸和30.0%(以还原糖计)的低聚糖。Ferulic acid esterase, arabinoxylanase, cellulase and Viscozyme L were used to prepare ferulic acid and oligosaccharide from maize bran. When 5 % cellulase and 5 % Viscozyme L （based on maize bran） were added to the reaction system containing one part of maize bran in 6 volume of mixed enzymes （w/v） prepared from A spergillus niger and reacted at pH5.5, 45 ℃ for 24 h, 25.4 % of ferulic acid and 30.0 % of oligosaccharide were released.

关 键 词：阿魏酸 低聚糖 玉米皮 酶解 

分 类 号：TQ914.1[化学工程]