碎米荠硒多糖的分离纯化及光谱分析  

Study on Purification and Spectra Analysis of Cardamine Urbaniana O.E.Schlz-Selenium Polysaccharide

作　　者：石宝霞[1] 车会莲[1] 赵利霞[2] 谭军[3] 潘思轶[3] 汪兴平[4] 柳玉[1] 

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]北京市食品酿造研究所,北京100050 [3]华中农业大学食品科技学院,湖北武汉430070 [4]湖北民族学院,湖北恩施445000

出　　处：《食品科学》2007年第6期298-302,共5页Food Science

摘　　要：本研究利用DEAE-Cellulose-52离子交换层析技术,采用步进式洗脱模式成功地将碎米荠硒多糖粗品进行Sephadex G-200葡聚糖凝胶柱层析,得到四种均一的硒多糖,SDS-PAGE电泳法鉴定其纯度。并采用紫外分光光度计、傅立叶变换红外光谱仪对硒多糖的结构进行了分析。DEAE-CeUulose-52 ion-exchange chromatography was used to separate CUS-SeP. It was successful to obtain four fractions, by the step gradient elution. PE was further purified by SephdexG-200. Electrophoresis was applied to identify the purity of PE, and identified to be electrophoresis grade purity. The structure characteristics of CUS-SeP were analysed with UV and FTIR.

关 键 词：硒多糖 分离纯化 红外光谱 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]