豆豉中大豆异黄酮及苷元降血糖活性及其机理的研究  

Study on Hypoglycemic Activity and Functional Mechanism of Soybean Isoflavone Glycone of Lobster Sauce

作　　者：郭瑞华[1] 霍文[1] 刘正猛[1] 吴少杰[1] 王和平[1] 王海龙[1] 梁彦云[1] 

机构地区：[1]华北煤炭医学院,河北唐山063000

出　　处：《时珍国医国药》2007年第7期1606-1607,共2页Lishizhen Medicine and Materia Medica Research

基　　金：河北省教委自然科学基金项目(No.2001136)

摘　　要：目的研究永川豆豉中大豆异黄酮及苷元的降血糖活性及其降血糖机制。方法永川豆豉粉碎,脱脂,然后用65%的乙醇浸泡,超声提取两次。所得提取液离心后旋转蒸发,用无水乙醇提取可得大豆异黄酮的粗提品。正丁醇饱和的水溶液除糖及色素等。然后经硅胶分离出大豆黄素和染料木素。河南鼠注射四氧嘧啶造成高血糖模型,连续7 d灌胃给予所提取的大豆异黄酮及苷元。用葡萄糖氧化酶法测定血糖水平。结果永川豆豉中的大豆异黄酮及苷元能显著地降低四氧嘧啶糖尿病模型鼠血糖水平,缓解糖尿病症状;大豆异黄酮和染料木素都能抑制葡萄糖的活性,尤以染料木素最为明显。结论豆豉中的大豆异黄酮可以明显地降低小鼠的高血糖,其所含的成分染料木素为主要的降血糖成分之一。Objective This article studied the activities and mechanism of Yongchuan fermented soybean isoflavone glycone to reduce the level of blood sugar. Methods After ground and removed of oil, Yongchuan fermented soybean was ultrasonic extracted with 65.% alcohol twice. The extraction was centrifuged. The liquid was rotate evaporated and then extracted with alcohol to obtain the crude extract. This crude extraction was removed of sugar and pigment with butanol saturated water and was separated by silica gel chromatography to yield dadezein and genestein. High blood sugar model was constructed by the injection of alloxan into Henan mice. The mice were treated with soya isoflavones for continuous 7 days. The blood sugar level was measured by GOD （glucose oxidase） method. Results The soya isoflavone glyeone from Yongchuan fermented soybean can remarkable reduce the level of blood sugar caused by alloxan, relaxing the symptoms of diabetes. Both crude extract of soya isoflavones from Yongchuan fermented soybean and genestein had the ability to inhibit the activity of α- glucosidase, with genestein more potent. Conclusion The soya isoflavones and isoflavone glycone from Yongchuan fermented soybean can obviously reduce the hyperglycemia of alloxan mice and the main component of reducing blood sugar may due to genestein.

关 键 词：豆豉 大豆异黄酮及苷元 Α-葡萄糖苷酶 染料木素 降血糖 

分 类 号：R285.5[医药卫生—中药学]