高效液相色谱法检测苦瓜啤酒中的苦瓜皂苷  

Detection of Bitter Melon Saponin in Bitter Melon Beer by HPLC

作　　者：李健[1] 刘鑫[1] 刘宁[1] 陈姝娟[1] 黄艳[1] 

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150076

出　　处：《食品科学》2007年第7期438-441,共4页Food Science

基　　金：黑龙江省自然科学基金重点项目(ZJN0606-02)

摘　　要：建立了高效液相色谱法对苦瓜啤酒中功效成分——苦瓜皂苷检测的方法。以70%乙醇超声辅助提取苦瓜皂苷,大孔树脂纯化,得苦瓜皂苷纯品。以自制的苦瓜皂苷为标准品与苦瓜啤酒中的苦瓜皂苷进行比较,用ODS-3柱,在205nm下,乙腈和水(55:45,V/V)为流动相,流速1.0ml/min,经反相液相色谱分析,以保留时间定性、峰面积定量。结果得出苦瓜啤酒中苦瓜皂苷的总含量为22.16μg/ml,相对标准偏差<4%,回收率在97.53%～100.32%之间。此方法重现性好且操作简单,是比较理想的检测方法。Detection of efficient component in bitter melon bccr was established by HPLC. The bitter melon saponin was extracted by transonogram-assistcd using 70% ethyl alcohol, then was purified by macroporous resin, and the straight of bitter melon saponin was obtained. Bitter melon saponin in bitter melon bccr was compared with standard substance made by our laboratory. An RP-HPLC method was set up, using ODS-3 column, acctonitrile： water （55：45, V/V） as mobile phase with a flow rate of 1.0 ml/min at the dctc^tion wavelength of 205 nm, the qualitative and the quantitative analyses wcrc carried out based on the retention time and peak area respectively. The results showed that the total content of bitter melon saponin in bitter melon beer is 22.16 μ g/ml, RSD （relative standard deviation） is less than 4%, recovery is between 97.53%~100.32%. This method is excellent reproducibility and the is operation simple. It is a perfect detection method.

关 键 词：高效液相色谱法 检测 苦瓜啤酒 苦瓜皂苷 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]