香椿叶抗氧化物提取工艺优化与抗氧化活性分析  

Extraction of Antioxidants from Toona Sinensis Leaves and Its Antioxidant Activities

作　　者：张京芳[1] 王冬梅[1] 周丽[1] 张强[1] 

机构地区：[1]西北农林科技大学林学院

出　　处：《农业机械学报》2007年第8期97-100,108,共5页Transactions of the Chinese Society for Agricultural Machinery

基　　金：陕西省科技攻关项目(项目编号:2005K03-G07);人事部留学人员科技活动择优资助项目

摘　　要：研究了香椿叶抗氧化物(ATSL)的最佳提取工艺及其抗氧化作用。以总抗氧化能力为指标,采用正交试验设计确定了ATSL的最佳提取条件是:以体积分数50%乙醇为提取溶剂,料液比0.1g/mL,80℃回流提取2次,每次3h。以Fenton反应、邻苯三酚自氧化和DPPH法检测其清除自由基活性,结果表明ATSL对·OH、O2-·和DPPH·的清除能力呈量效关系。从ATSL对不同抗氧化反应的IC50看,其对DPPH·的清除能力大于对·OH和O2-·的清除能力。用硫氰酸铵法评价ATSL在亚油酸乳状体系中的抗脂质过氧化作用,发现0.05g/LATSL抗脂质过氧化作用比相同浓度的BHT弱,比L-抗坏血酸强,与α-生育酚相当。The aim of the study was to obtain crude antioxidants from toona sinensis leaves （ATSL） and evaluate its anti-oxidative activity. The optimum extracting condition was investigated by using orthogonal test with total anti-oxidative capacity （TAC） as index, and as follows： 50% ethanol as solvent, ratio of material to liquor was 0.1 g/mL, at 80 C for 3 h, and extracted for two times. The anti-oxidative activities, including hydroxyl radicals, super oxide radicals and 1,1- dipheny free rad -2-picrylhydrazyl （DPPH） radicals were studied. ATSL showed concentration-dependent cals scavenging activity. From a comparison of the C50 values for different anti-oxidative reactions, it seemed that ATSL was more effective in scavenging DPPH radicals than scavenging hydroxyl radicals and superoxide radicals. In linoleic acid emulsion, ATSL exhibited less inhibitory effect on lipid peroxidation as assayed by the ammonium thiocyanate method than BHT but stronger than L-ascorbic acid, and matched α-tocopherol at the same concentration of 0. 05 g/L.

关 键 词：香椿叶 抗氧化物 提取工艺 正交试验 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]