一步酸法水解菊粉生产高果糖浆  

Production of High-Fructose Syrup by Inulinase from Jerusalem Artichokes

作　　者：谢秋宏[1] 相宏宇[2] 

机构地区：[1]吉林大学分子生物学系,长春130023 [2]白求恩医科大学预防医学院,长春130021

出　　处：《吉林大学自然科学学报》1997年第2期103-105,共3页Acta Scientiarum Naturalium Universitatis Jilinensis

摘　　要：用黑曲霉突变株AJ1958产生的菊粉酶，水解菊粉抽提液生产高果糖浆．以6O目菊芋粉在水料比为6：1，料液pH3．0~3．5，60℃条件下浸泡0·5h，过滤，其菊粉抽提率可达95％以上．在每克菊粉投酶量120μmol／min下，水解菊粉抽提液6h～7h，水解率达到95％以上．调nH至4．2～4．5，经活性炭脱色，减压浓缩，得可溶性固形物浓度为0·77g／g以上，果糖含量0．90g／g以上，具有果糖纯正香味的高果糖浆．The comminuted tubers of jerusalem artichokes were extracted with 6 volumes of water for 0. 5 h at 60℃ and at the pH range of 3. 0~3. 5. The rate of inulin extraction reached up to 95 %. The extracting solution was hydrolyzed for 6 h^7 h at 60℃ and at pH 3. 0~3. 5 under adding 120 μmol/(min. g) inulin of inulinase. The rate of hydrolysis was over 95%. The hydrolysate was increased at pH 4. 2~4. 5 and decolorized by activated charcoal and concentrated. The high-fructose syrup (HFS)was producted. The fructose concent of the HFS is over 0. 90 g/g.

关 键 词：菊粉 菊粉酶 高果糖浆 一步酶法 水解 

分 类 号：TQ464.1[化学工程—制药化工]