桑黄黄酮高产菌株深层发酵条件的优化  

Optimization of submerged fermentation parameters for high-yield flavone-producing strain of Phellinus baumii

作　　者：赵子高[1,2] 杨焱[1] 刘艳芳[1] 张劲松[1] 唐庆九[1] 潘迎捷[3] 

机构地区：[1]上海市农业科学院食用菌研究所 [2]南京农业大学生命科学学院,江苏南京210095 [3]上海水产大学食品科学学院

出　　处：《中国酿造》2007年第9期22-25,共4页China Brewing

基　　金：上海市科委农医重点攻关项目(063919143)

摘　　要：以菌丝体黄酮含量、产量及胞外黄酮产量为评价指标,对桑黄(鲍氏层孔菌)产黄酮发酵条件进行了优化,通过单因素及4因素3水平正交试验筛选出了桑黄黄酮发酵的最适培养基:葡萄糖10g/L,玉米粉40g/L,豆饼粉5g/L,KH2PO43g/L,MgSO41.5g/L。通过优化培养条件,得到最适产黄酮液体发酵条件为培养温度28℃,摇床转速160r/min,pH值自然,接种量8%～10%,装液量100mL(250mL三角瓶),发酵周期144h。Using the flavone yields of Phellinus baumii in vivo and in vitro as evaluation indices, the optimal submerged fermentation parameters for flavone production were investigated. With single factor analysis and L9 （3^4） orthogonal test, the optimal medium for fermentation was established as： glucose 10 g/L, corn flour 40 g/L, soybean cake powder 5 g/L, KH2PO4 1.5 g/L and MgSO4 1 g/L. The optimal fermentation conditions for flavone production were described as： culture temperature 28%, rotation speed 160 r/min, natural pH, inoculation size 8%-10%, liquid volume 100 ml in 250ml Erlenmeyer flask, and fermentation time 144 h.

关 键 词：桑黄 类黄酮 深层发酵 

分 类 号：TS202.3[轻工技术与工程—食品科学]