高效液相色谱电喷雾质谱联机检测黑豆异黄酮  

Identification and Determination of Black Bean Isoflavones by High Performance Liquid Chromatography Coupled with Electrospray Ionization Mass Spectrum

作　　者：李丹[1] 牟莉[1] 李晓磊[1] 谭克[1] 

机构地区：[1]长春大学吉林省农产品加工重点实验室

出　　处：《食品科学》2007年第9期438-441,共4页Food Science

基　　金：国家科技成果重点推广计划项目(2003EC000127);吉林省科技发展计划项目(20050125)

摘　　要：采用C18色谱柱,以甲醇:水:甲酸30min内,0.8ml/min梯度洗脱(从20:80:0.1到80:20:0.1)黑豆70%乙醇提取液。紫外光谱和电喷雾质谱的数据表明:大豆苷、乙酰基染料木苷、染料木苷、丙二酰基大豆苷、丙二酰基黄豆苷和丙二酰基染料木苷是黑豆种子中最主要的异黄酮,总含量为0.373%,为普通大豆种子异黄酮含量的2～6倍;黑豆是异黄酮的丰富来源。Mobile phases of 100% methanol/water/formic acid from 20：80：0.1 to 80：20：0.1 respectively in 30 min were used to elute black bean isoflavones at 0.8 ml/min,extracted by 70% ethanol from mature black bean seeds, in a C18 colurnn with a high performance liquid chromatography （HPLC） system coupled with diode array detector （DAD） and mass spectrum （MS）. Daidzin, acetyl genistin, genistin, malonyl daidzin, malonyl glycitin and malonyl genistin are identified as the main isoflavones with a total content 0.373%. Black bean is a good source of isoflavones for human health.

关 键 词：黑豆 异黄酮 高效液相色谱 质谱 紫外光谱 

分 类 号：TS201[轻工技术与工程—食品科学]