玉米抗性淀粉酶解法制备工艺的研究  

Enzymolysis Technology for Preparation of Corn Resistant Starch

作　　者：张守文[1] 孟庆虹 杨春华[1] 赵凯[1] 

机构地区：[1]哈尔滨商业大学食品工程学院,哈尔滨150076 [2]黑龙江省农科院,哈尔滨150080

出　　处：《中国粮油学报》2007年第5期53-56,共4页Journal of the Chinese Cereals and Oils Association

基　　金：国家自然科学基金资助项目(30271117);教育部重点资助项目(204042)

摘　　要：以抗性淀粉得率为评价指标,采用酶解法制备玉米抗性淀粉,通过正交试验确定了酶解法制备的最佳工艺条件:α-淀粉酶酶解条件为淀粉乳浓度20%,α-淀粉酶用量15u/g,酶解温度70℃;普鲁兰酶脱支条件为普鲁兰酶用量4u/g,脱支时间10h,pH值4.5;糊化条件为糊化时间20min,糊化温度120℃。The corn resistant starch was prepared by using the enzymolysis method with the resistant starch yield as evaluation index. The conditions of the enzymolysis method were optimized by an orthogonal experiment. The obtained conditions for the α - amylase enzymolysis are starch milk concentration 20%, α - amylase dosage 15u/g, and enzymolysis temperature 70℃ ; those for the enzymatic debranching by Promozyme are Promozyme dosage 4u/g, debranching time 10h, and pH 4.5 ; those for the gelatinization are gelatinization time 20min and gelatinization temperature 120 ℃.

关 键 词：玉米抗性淀粉 Α-淀粉酶 普鲁兰酶 制备 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]