代谢过程中相关氨基酸对高密度发酵生产腺苷蛋氨酸的影响  

Influences of amino acids on high-cell-density fermentation for S-adenosyl-L-methionine production

作　　者：刘沛溢[1] 王杰鹏[1] 谭天伟[1] 

机构地区：[1]北京化工大学生命科学与技术学院北京市生物加工过程重点实验室,北京100029

出　　处：《生物加工过程》2007年第4期48-53,共6页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20306002);国家高技术研究发展计划(863)资助项目(2002AA217022)

摘　　要：对清酒酵母高密度发酵生产S-腺苷-L-蛋氨酸(SAM)代谢过程中的相关氨基酸进行了考察。分别考察了十二种氨基酸对生物量和SAM产量的影响。实验发现L-胱氨酸、L-半胱氨酸、L-赖氨酸、L-组氨酸和L-蛋氨酸对SAM的积累有利,其中L-赖氨酸和L-组氨酸可以提高生物量,进而提高SAM的产量;L-胱氨酸、L-半胱氨酸和L-蛋氨酸可以提高SAM的含量,但是会抑制生物量的增长。通过3种补加方式的比较,得到最优的补加方式为:L-赖氨酸和L-组氨酸在培养基中加入,L-胱氨酸,L-半胱氨酸和L-蛋氨酸采取在发酵过程前24 h流加。通过正交实验确定补加量为:L-赖氨酸为1 g/L,L-组氨酸为1 g/L,L-胱氨酸为1.5 g/L,L-半胱氨酸为1 g/L,L-蛋氨酸为1 g/L。将此结果应用于5 L发酵罐培养,SAM最高产量为5.53 g/L,生物量为128 g/L。The influences of twelve amino acids on S-adenosyl-L-methionine （SAM） production by Saccharomyces cerevisiae were investigated. The results showed that L- cystine, L-cysteine, L- lysine, L-his- tidine and L- methionine enhanced the production of SAM. Among them, L-lysine and L-histidine increased the biomass,thus increas the production of SAM. The others three promoted the concentration of SAM but had negative effects on biomass. Three different feeding methods were studied. It was found that L-lysine and L-histidine should be added in the cultural media, and the other should be fed during the first 24. The amounts of the five aminu avids were 1 g/L, 1 g/L, 1.5 g/L, 1 g/L and 1 g/L respectively according to orthogonal optimization. Experiment on 5 L fermenter revealed that the accumulation of SAM reached 5.53 g/L,and 128 g/L of biomass was obtained.

关 键 词：S-腺苷-L-蛋氨酸 氨基酸 高密度发酵 清酒酵母 

分 类 号：TQ929[轻工技术与工程—发酵工程]