猪肉中16种性激素的多残留检测方法  

Determination of 16 Sex Hormones in Pork by Gas Chromatography-mass Spectrometry

作　　者：湛嘉[1] 李佐卿[1] 俞雪钧[1] 樊苑牧[1] 陈树兵[1] 谢东华[1] 房科腾[1] 杨娟芬[1] 

机构地区：[1]宁波出入境检验检疫局,浙江宁波315012

出　　处：《宁波大学学报（理工版）》2007年第4期520-525,共6页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：国家质检总局项目(2005IK033)

摘　　要：采用安捷仑GC/MS5975N能同时对猪肉中16种性激素残留进行筛选、定量和确证.对5.0g肌肉样品的检出限均在0.25～1.0μg?kg-1之间.猪肉的平均回收率分别为83.7%～107.9%,平均变异系数为6.8%～19.7%.当添加水平为1.0μg·kg-1时,本方法不同批次猪肉回收率的相对标准偏差为7.7%～21.6%,重复性好.另外,与安捷仑GC/MS5973分析相比,采用GC/MS5975N时,雌三醇衍生物定量离子为m/z 876,比m/z 663峰度强。Using GC/MS （Agilent 5975N）, the author proposes a highly efficient method for simultaneous determination of 16 sex hormones in pork. In analytical process the detection limits are 0.25-μg·kg^-1. Overall recovery （%） and relative standard deviations（RSD, %） range from 83.7% to 107.9%, and from 6.8% to 19.7 %, respectively. The within-laboratory repeatability （RSD, %） range from 7.7% to 21.6% when the adding level of 1.0 μg·kg^-1 is used. The results show the satisfactory recovery and repeatability. In addition, when GC/MS（Agilent 5975N） is used, the quantifier ion of heptafluorobutyric anhydride derivative of estriol is m/z 876 whose intensity is higher than that ofm/z 663.

关 键 词：性激素 气相色谱-质谱联用 猪肉 

分 类 号：TS201.6[轻工技术与工程—食品科学]