油脂降解菌的选育与鉴定  

作　　者：闫亚娟[1] 李宗伟[1] 李宗义[1] 刘建玲[2] 秦广雍[1] 朱海霞[1] 汪杏莉[1] 

机构地区：[1]郑州大学离子束生物工程省重点实验室,河南郑州450052 [2]西北大学生命科学学院,陕西西安710069

出　　处：《食品工业科技》2007年第12期57-60,共4页Science and Technology of Food Industry

基　　金：国家"973"资助项目(2002CB412308)

摘　　要：从学校餐厅下水道口、油脂加工厂及屠宰场的含油废水及土壤中采集6个土样,根据油脂平板上是否有水解圈及水解圈的大小得到9株菌,复筛结果显示C-3-2菌株的降解率最大。通过对该酵母菌进行形态学观察及生理生化特征的测试,初步鉴定为假丝酵母属(Candidaspp)。在此基础上进行了离子束诱变,以筛选出的Candidaspp为出发菌株,经离子束辐照后得到一株高降解率的菌株。结果表明:离子束诱变后,其降解率提高了11.09%。Six oil- contained sewage and soil samples were collected from cloaca of university dining-room, oil manufactory, slaughterhourse.According to hydrolytic circle, 9 strains were isolated. Oil-degrading rate of C-3-2 strain was the biggest after secondary screening. Utilizing the morphology, physiology and biochemistry methods, the yeast was identified as Candidaspp. On the base of this, the original strain Candidaspp was implanted by ion beam, and a higher strain of oil-degrading rate was obtained.The results showed that the oil- degrading rate was increased by 11.09%.

关 键 词：脂肪酶 离子束 酵母菌 假丝酵母属 

分 类 号：TS201.3[轻工技术与工程—食品科学]