酶制剂在大曲丢糟再发酵中的应用  

Application of Enzyme in the Refermentation with Daqu Distiller's Grain

作　　者：李新社[1] 陆步诗[1] 伍翠兰[1] 

机构地区：[1]邵阳学院生物与化学工程系,湖南邵阳422000

出　　处：《生物技术》2008年第1期76-78,共3页Biotechnology

基　　金：湖南省教育厅项目资助(编号:04C606)

摘　　要：目的:在添加糖化酶和纤维素酶的条件下,采用黑曲霉与假丝酵母发酵丢糟生产单细胞蛋白饲料。方法:采用L9(34)水平正交试验对糖化酶与纤维素酶的添加量、黑曲霉与假丝酵母的比例、发酵时间进行了探讨。结果:单细胞蛋白饲料生产的最佳生产工艺条件为:糖化酶的添加量为0.03%、纤维素酶的添加量为1.5%、黑曲霉∶假丝酵母=1∶3、培养时间为8d,产品粗蛋白含量可达38.72%。结论:酶制剂的添加对丢糟转化为单细胞蛋白饲料具有明显的促进作用。Objective： Aspergillus niger and Candida mycoderma was used to ferment Daqu distiller＇s grain to produce single cell protein feed with the additive of glucoamylase and cellulose.Methods： The additive amount of glucoamylase and celulase , the ratio of Aspergillus ruger and Candida mycoderma , the fermention time were studied by L9 （3^4）orthogonal test. Results： The best production conditions of single cell protein feed were obtained. The additive amount of glucoamylase is 0.03%, the additive amount of celulase is 1.5% ,the ratio of Aspergtillus ruger and Candida mycoderma is 1 ： 3, the fermention time is eight days. The contents of rough protein was increased up to 38.73 %. Conclusion： The additive of gtucoamylase and cellulose have obviously action which promote single cell protein feed transform from the distiller＇s grain.

关 键 词：丢糟 糖化酶 纤维素酶 饲料 黑曲霉 假丝酵母 再发酵 单细胞蛋白 

分 类 号：S816.4[农业科学—饲料科学]