Protamex复合蛋白酶酶解珠蛋白的研究  

Study on hydrolyzing the globin by Protamex protease

作　　者：陆利霞[1] 宫国君[1] 孙芸[1] 熊晓辉[1] 

机构地区：[1]南京工业大学制药与生命科学学院,南京210009

出　　处：《食品科技》2008年第2期60-62,共3页Food Science and Technology

摘　　要：以酸化丙酮法从猪血中提取的珠蛋白为原料,探讨了酶用量、珠蛋白浓度、温度、pH值及作用时间对水解度的影响。结果表明,Protamex复合蛋白酶水解珠蛋白的适宜条件为pH6.5,温度为45℃,Protamex复合蛋白酶用量为0.0915 Au/g,底物浓度3%,水解时间6h。改善了珠蛋白在中性条件下的溶解性。Globin was prepared from porcine blood by the acid-acetone method. Protamex protease was used to hydrolyze the globin. The effects of different pH, temperature, substrate concentration, protease con- centration and the time on hydrolyzing globins were researched. The results showed the optimum hydrolyzed condition of globin by Protamex protease was protease concentration 0.0915 Au/g, temperature 45℃, pH 6.5, globin concentration 3% for 6 h. And the solution of hydrolyzing globins was improved.

关 键 词：PROTAMEX复合蛋白酶 珠蛋白 猪血 

分 类 号：TS201.2[轻工技术与工程—食品科学]