乳酸片球菌抑菌物质细菌素的分离纯化  

Extraction and Purification of Bacteriocin from Pediococcus acidilactici Strain

作　　者：李亚玲[1] 周志江[2] 韩烨[2] 王培[1] 丁成为[1] 白鹤[1] 

机构地区：[1]天津大学化工学院,天津300072 [2]天津大学农业与生物工程学院,天津300072

出　　处：《食品科学》2008年第2期271-274,共4页Food Science

基　　金：国家自然科学基金项目(30571378)

摘　　要：将乳酸片球菌接种于MRS培养基,37℃静置培养17h后产生抑菌物质细菌素。用三步法从培养液中分离纯化细菌素:首先根据细菌素在pH6.0时与菌体的吸附力最强,pH2.0时吸附力最弱,通过调节pH和离心来收集细菌素,然后经过SP Sepharose Fast Flow阳离子交换柱层析和Sephadex G-50凝胶层析进行纯化,最后细菌素的得率为0.19%,比活为5.17×104AU/mg,纯化了34.54倍。得到的细菌素样品通过Tricine-SDS-PAGE电泳出现清晰条带,大小约为4600D。Fresh culture of Pediococcus acidilactici strain inoculated in MRS broth and cultivated under 37℃ for 17 h, the bacteriocin was extracted from the culture and purified by three steps： the pH mediated cel/adsorption-desorption method, SP Sepharose Fast Flow ion-exchange chromatography and Sephadex G-50 gel filtration. The final yield of bacteriocin was 0. 19% and the specific activity was 5.17 × 10^4 AU/mg. The specific activity was increased by 34.54 fold. Tricine-SDS-PAGE showed that its molecular weight was about 4600 D.

关 键 词：细菌素 分离 纯化 Tricine-SDS-PAGE 

分 类 号：R996.1[医药卫生—毒理学]