Ameliorative effects of bombesin and neurotensin on trinitrobenzene sulphonic acid-induced colitis,oxidative damage and apoptosis in rats  

作　　者：Alper Akcan Sebahattin Muhtaroglu Hulya Akgun Hizir Akyildiz Can Kucuk Erdogan Sozuer Alper Yurci Namik Yilmaz 

机构地区：[1]Department of General Surgery, ErciyesUniversity School of Medicine, Kayseri 38039, Turkey [2]Department of Biochemistry, ErciyeUniversity School of Medicine, Kayseri 38039, Turkey [3]Department of Pathology, Erciyes University School of Medicine, Kayseri 38039, Turkey [4]Department of Gastroenterology, Erciyes University School of Medicine, Kayseri 38039, Turkey

出　　处：《World Journal of Gastroenterology》2008年第8期1222-1230,共9页世界胃肠病学杂志（英文版）

基　　金：Grant (SBAG-105S338) from the Scientific and Technical Research Council of Turkey (TUBITAK)

摘　　要：AIM:To investigate the effects of bombesin (BBS) and neurotensin (NTS) on apoptosis and colitis in an ulcerative colitis model. METHODS:In this study, a total of 50 rats were divided equally into 5 groups. In the control group, no colitis induction or drug administration was performed. Colitis was induced in all other groups. Following the induction of colitis, BBS, NTS or both were applied to three groups of rats. The remaining group (colitis group) received no treatment. On the 11th d after induction of colitis and drug treatment, blood samples were collected for TNF-α and IL-6 level studies. Malondialdehyde (MDA), carbonyl, myeloperoxidase (MPO) and caspase-3 activities, as well as histopathological findings, evaluated in colonic tissues. RESULTS:According to the macroscopic and microscopic findings, the study groups treated with BBS, NTS and BBS + NTS showed significantly lower damage and inflammation compared with the colitis group (macroscopic score, 2.1 ± 0.87, 3.7 ± 0.94 and 2.1 ± 0.87 vs 7.3 ± 0.94;microscopic score, 2.0 ± 0.66, 3.3 ± 0.82 and 1.8 ± 0.63 vs 5.2 ± 0.78, P < 0.01). TNF-α and IL-6 levels were increased significantly in all groups compared with the control group. These increases were significantly smaller in the BBS, NTS and BBS + NTS groups compared with the colitis group (TNF-α levels, 169.69 ± 53.56, 245.86 ± 64.85 and 175.54 ± 42.19 vs 556.44 ± 49.82;IL-6 levels, 443.30 ± 53.99, 612.80 ± 70.39 and 396.80 ± 78.43 vs 1505.90 ± 222.23, P < 0.05). The colonic MPO and MDA levels were significantly lower in control, BBS, NTS and BBS + NTS groups than in the colitis group (MPO levels, 24.36 ± 8.10, 40.51 ± 8.67 and 25.83 ± 6.43 vs 161.47 ± 38.24;MDA levels, 4.70 ± 1.41, 6.55 ± 1.12 and 4.51 ± 0.54 vs 15.60 ± 1.88, P < 0.05). Carbonyl content and caspase-3 levels were higher in the colitis and NTS groups than in control, BBS and BBS + NTS groups (carbonyl levels, 553.99 ± 59.58 and 336.26 ± 35.72 vs 209.76 ± 30.92, 219.76 ± 25.77 and 220.34 ± 36.95;caspase-3 levelAIM： TO investigate the effects of bombesin （BBS） and neurotensin （NTS） on apoptosis and colitis in an ulcerative colitis model.METHODS： In this study, a total of 50 rats were divided equally into 5 groups. In the control group, no colitis induction or drug administration was performed. Colitis was induced in all other groups. Following the induction of colitis, BBS, NTS or both were applied to three groups of rats. The remaining group （colitis group） received no treatment. On the 11th d after induction of colitis and drug treatment, blood samples were collected for TNF-α and IL-6 level studies. Malondialdehyde （MDA）, carbonyl, myeloperoxidase （MPO） and caspase-3 activities, as well as histopathological findings, evaluated in colonic tissues.RESULTS： According to the macroscopic and microscopic findings, the study groups treated with BBS, NTS and BBS ＋ NTS showed significantly lower damage and inflammation compared with the colitis group （macroscopic score, 2.1 ± 0.87, 3.7 ± 0.94 and 2.1 ± 0.87 vs 7.3 ± 0.94; microscopic score, 2.0 ± 0.66, 3.3 ± 0.82 and 1.8 ± 0.63 vs 5.2 ± 0.78, P 〈 0.01）. TNF-α and IL-6 levels were increased significantly in all groupscompared with the control group. These increases were significantly smaller in the BBS, NTS and BBS ＋ NTS groups compared with the colitis group （TNF-α levels, 169.69 ± 53.56, 245.86 ± 64.85 and 175.54 ± 42.19 vs 556.44 ± 49.82; IL-6 levels, 443.30 ± 53.99, 612.80 ± 70.39 and 396.80 ± 78.43 vs 1505.90 ± 222.23, P 〈 0.05）. The colonic MPO and MDA levels were significantly lower in control, BBS, NTS and BBS ＋ NTS groups than in the colitis group （MPO levels, 24.36 ± 8.10, 40.51 ± 8.67 and 25.83 ± 6.43 vs 161.47 ± 38.24; MDA levels, 4.70 ± 1.41, 6.55 ± 1.12 and 4.51 ± 0.54 vs 15.60 ± 1.88, P 〈 0.05）. Carbonyl content and caspase-3 levels were higher in the colitis and NTS groups than in control, BBS and BBS ＋ NTS groups （carbonyl levels, 553.99 ± 59.58 and 336.26 ± 35.72 vs 209.76 ± 3

关 键 词：BOMBESIN NEUROTENSIN COLITIS APOPTOSIS 

分 类 号：R574.62[医药卫生—消化系统]