离子注入右旋糖酐生产菌的诱变效应研究  

STUDY OF THE MUTAGENIC EFFECT OF THE ENERGETIC ION IMPLANTATION TO THE DEXTRAN PRODUCING STRAIN

作　　者：罗大珍[1] 朱文 马继霞[1] 王宇钢[2] 赵渭江[2] 

机构地区：[1]北京大学生命科学学院,北京100871 [2]北京大学重离子物理研究所,北京100871

出　　处：《微生物学报》1997年第4期312-315,共4页Acta Microbiologica Sinica

基　　金：核工业科学基金资助项目

摘　　要：离子注入作为一项新的生物诱变技术是近年发展起来的,已引起国内外学者的极大关注。80年代中期,我国学者已将低能离子注入技术应用于农作物诱变育种研究中,取得了明显的效果。这对以后的拓宽诱变源、创造广泛的变异类型、选育高产优良的新品种,具有重大的理论意义和实用价值。Using ion implantation with H+ of 2.4 meV produced from 4.5 mV electrostatic accelerator, we studied the mutagenic effect on the dextran producing strain-Leucons toe mescnteroides in aqueous solution. The survival curve of the primary implanted strain was obtained in the ion dose ranging from 5×1011 ions/cm2 to 1 ×1014 ions/cm2. After ion implantation, more than one hundred mutant strains were selected. It was found that the production of dextray with large deviation from the control, about the 90% selected strain, were lower than that of the control, but other 10% were higher. Twenty strains were further screened, two mutant strains were selected. One of its productions was 11.5% and the other was 36.5% higher than that of the control. The production of the dextran anhydride maintained stable after six times of continuous transplantation of the culture. We found that the growth rate of a large part of the implanted strains was slower and the growth period was postponed because of the damage induced by energetic ion bombardment. From the SEM (scanning electronic microscopy) observation, the form of the implanted strains was changed indicating the cell damage was due to the ion induced mutagenic effect.

关 键 词：右旋糖酐 肠膜状明串珠菌 离子注入 诱变 发酵 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ929