固定化酵母细胞发酵玉米芯酶解液生产木糖醇  

Xylitol Production by Immobilized Yeast Cell Using Enzymatic Hydrolysate of Corncob

作　　者：张朝晖[1] 董丽辉[2] 周晓云[1] 

机构地区：[1]浙江工业大学生物与环境工程学院生物工程系,浙江杭州310014 [2]浙江医药高等专科学校,浙江宁波315100

出　　处：《纤维素科学与技术》2008年第1期1-6,共6页Journal of Cellulose Science and Technology

基　　金：浙江省自然科学基金资助项目(302067)

摘　　要：用木聚糖酶对玉米芯的自水解液进行酶解以获得可发酵的木糖溶液。该法与直接用酶对玉米芯水解相比,水解速度快,木糖得率较高。所得酶解液发酵木糖醇的性能虽不如纯木糖,但明显优于玉米芯酸水解液。海藻酸钙/壳聚糖(ACA)微胶囊的最佳成膜时间和液化时间分别是18 min和20 min,微胶囊使用的环境pH范围是3～6。用微胶囊固定化细胞发酵玉米芯酶解液,重复培养了8批,平均木糖醇得率为61.4%。The comcob auto-hydrolysate was hydrolyzed by xylanase to obtain the fermentable xylose solution. Its hydrolysis rate was faster and yield of xylose was higher than the direct hydrolyzing corncob by xylanase. Although the fermentability of the enzymatic hydrolysate by yeast was not as good as pure xylose solution, it was much better than the acid hydrolysate of corncob. In making alginate/chitosan （ACA） microcapsules, the optimal reaction time and liquefying time was 18 and 20 min respectively. The microcapsules should be used at pH 3-6. The immobilized cells by ACA microcapsules were used to produce xylitol using enzymatic hydrolysate in repeated batch cultures. The average yield of xylitol reached 61.4% in 8 batches.

关 键 词：木聚糖酶 玉米芯 酶解液 固定化细胞 木糖醇 

分 类 号：TQ353.421[化学工程]