超声辅助提取紫苏叶黄酮及其清除自由基作用研究  

Ultrasonic-aided Extraction of Flavonoids from Perilla frutescens(L.) Britt Leaves and Evaluation for Free-radical Scavenging

作　　者：刘宁[1] 仇农学[1] 田玉霞[1] 

机构地区：[1]陕西师范大学食品工程系,陕西西安710062

出　　处：《西北林学院学报》2008年第1期158-161,共4页Journal of Northwest Forestry University

摘　　要：紫苏是传统的药食同源植物,为进一步研究其生物活性成分,以乙醇溶液浸提,结合超声波辅助方法,采用L9(34)正交试验设计研究了紫苏叶黄酮的最佳提取工艺,并测定了其对羟自由基(.OH)和1,1-二苯基-2-苦苯肼自由基(DPPH.)的清除效果。结果表明,黄酮最佳提取工艺条件为:50%乙醇溶剂,料液比1∶30,提取时间60 m in,提取温度60℃,该条件下总提取率可达6.82%。在一定范围内,提取物对.OH的清除效果与其浓度呈线性关系(R2=0.994 7),IC50为0.130 mg/mL;对DPPH.有极强清除作用,其IC50为0.032 mg/mL。试验表明紫苏叶含有丰富的黄酮类物质,其具有体外抗氧化性,是一种有效的天然自由基清除剂,具有很大的开发利用前景。Perilla frutescens （L.） Britt was a kind of traditional nutriceutical. In order to investigate its bioactivity components, the optimum conditions of extracting flavonoids from P. frutescens） leaves by ultrasonic-aided and ethanol extraction were studied by using L9（34） orthogonal method. Meanwhile, free- radical scavenging capacities were investigated. The results showed that the optimal extraction condition were solvent 50% ethanol, the ratio of material to solvent was 1 ： 30, and time of extraction 60 min, temperature 60℃. In these conditions, the total extracting rate was as high as 6. 82%. Furthermore, a line relationship existed between scavenging of hydroxyl radical and the flavonoids in perilla leaves （R^2= 0.9947）, the IC50 （inhibitory concentration 50%） being 0. 130 mg/mL. The flavonoids also showed very excellent inhibitory effects on 1,1-dipheny 1-2-picrylhydrazyl radical, and the IC50 was 0. 032 mg/mL. The test indicated that there were abundant flavonoids in P. frutescens leaves which were effective natural free-radical scavenging reagents, and they had a great exploiting foreground.

关 键 词：紫苏叶 生物黄酮 超声波辅助提取 清除自由基 

分 类 号：S789.4[农业科学—林学]