里氏木霉摇瓶发酵产木聚糖酶培养条件的优化  

Optimization of flask liquid fermentation medium for xylanase producing strain Trichoderma reesei

作　　者：魏瑛[1] 童群义[1] 李博[2] 

机构地区：[1]江南大学食品学院,无锡214122 [2]西北农林科技大学食品科学与工程学院,杨凌712100

出　　处：《安徽农业大学学报》2008年第2期271-274,共4页Journal of Anhui Agricultural University

摘　　要：研究培养基组分及培养条件对里氏木霉M（Trichoderma reesei）摇瓶发酵产木聚糖酶的影响。将里氏木霉M在培养温度30℃，转速为200r·min。条件下培养，采用分批正交试验设计确定最佳培养基组成为牛肉膏、蛋白胨、玉米芯、葡萄糖，其比例为1：0．50：3：0．43，最适培养时间为64～72h，最适初始pH为5。Trichoderma reesei was cultured in different medium to study the ability of producing xylanase. Trichoderma reesei was cultured at 30℃ with rotate speed at 200 r· min^- 1, the optimum medium was determined as follows：beef extract： peptone： corncobs： glucose for 1： 0. 50： 3： 0. 43. After studying the fermentation in flask to determinat the optimum culture time and pH, 64 -72 h, pH 5 were determined to be the optimum conditions.

关 键 词：里氏木霉 木聚糖酶 正交试验 

分 类 号：Q55[生物学—生物化学]