随机扩增多态性DNA技术在沙门氏菌同源性分析中的应用  

Application of RAPD in Homology Analysis of Salmonella

作　　者：郭爱玲[1] 谢跻[1] 粟婉媛[1] 王洪江[1] 柳婷[1] 汪胜[1] 马美湖[1] 

机构地区：[1]华中农业大学食品科技学院,湖北武汉430070

出　　处：《食品科学》2008年第4期355-357,共3页Food Science

摘　　要：目的:利用随机扩增多态性DNA技术(random amplified polymorphicDNA,RAPD)对从食品、蛋品样本或从业人员中收集到的67个地方菌株、4个模式菌株,涉及29种血清型的沙门氏菌进行同源性分析,为沙门氏菌食源性疾病的流行病学溯源和疫情控制提供理论依据。方法:选用筛选得到的10个碱基引物(CCGAAGCTGC)运用RAPD技术对不同来源的71株沙门氏菌进行随即引物扩增,得到的RAPD图谱使用NTSYS-pc2.10e软件进行聚类分析。结果:相似性系数在0.70时,71株沙门氏菌可以分为七大类群,其中最多的一群内聚集的菌株达54株,相似性系数在0.90时,可以分为26个群,最多的一群内聚集的菌株达18株,相似性系数为1.00时,有9个组群,最多的一组群内聚集的菌株达11。同源性结果和血清学鉴定的结果有90%的吻合率。结论:RAPD检测技术可以用于沙门氏菌食源性疾病的流行病学调查和溯源。Objective： 67 local strains isolated from food samples and egg samples and 4 type strains were analyzed by random amplified polymorphic DNA（RAPD）. It was not only related with the homology analysis of 29 Serotype of Salmonella, but also provided basis for the epidemiological traceability of food-borne disease and the controlling of the disease, Method： 10 bases primer were selected in the use of the RAPD analysis of 71 Salmonella. The fingerprinting of RAPD result was analyzed by NTSYSpc2.10e. Result： When the rate of correlation is 0.7, 71 Salmonella can be divided into 7 groups. The group of most number has 54 strains. When the rate of correlation is 0.9, 71 Salmonella can be divided into 26 groups. The group of most number has 18 strains. When the rate of correlation is 1.0, 9 groups can reach the level, The group of most number has 11 strains. The result of RAPD analysis agrees with the analysis of serological test at the level of 90 percent. Conclusion： the technique of RAPD can be used to the epidemiological traceability of food-borne disease.

关 键 词：沙门氏菌 地方菌株 RAPD 聚类分析 同源性 

分 类 号：TS207.4[轻工技术与工程—食品科学]