普洱茶提取物对高糖作用下人胚肺成纤维细胞的保护作用  

Protective Effect of Pu-erh Tea Extracts on High-glucose-induced Damage in Human Fibroblast Cells

作　　者：揭国良[1] 何普明[1] 张龙泽[2] 赵保路[2] 吕海鹏[3] 林智[3] 

机构地区：[1]浙江大学茶叶研究所,浙江杭州310029 [2]中国科学院生物物理研究所,脑与认知国家重点实验室,北京100101 [3]中国农业科学院茶叶研究所,浙江杭州310008

出　　处：《食品科学》2008年第4期366-369,共4页Food Science

基　　金：国家自然科学基金项目(30370367)

摘　　要：本研究采用丙酮、水、氯仿、乙酸乙酯和正丁醇连续萃取普洱茶,利用柱色谱分离萃取物。研究结果表明,普洱茶乙酸乙酯萃取物的E2、E8组分,正丁醇萃取物的B2和B5组分具有很强的超氧阴离子自由基和羟自由基清除能力,对高浓度葡萄糖作用下的人胚肺成纤维细胞HPF-1的损伤具有很强的保护作用。In this study, the Pu-erh tea was successively extracted with acetone, water, chloroform, ethyl acetate, and n-butanol. The extracts were then isolated by column chromatography. Our study demonstrated that both the Fraction 2 （E2 and）, Fraction 8 （E8） from ethyl acetate extract, and the Fraction 4 （B4） and, Fraction 5 （B5） from n-butanol extract have strong superoxide anions and hydroxyl radicals scavenging activity. The four fractions showed above have protective effects against high concentration glucose-induced loss of HPF-1 cell viability.

关 键 词：茶 普洱茶 自由基 天然抗氧化剂 抗氧化 HPF-1细胞 

分 类 号：TS201[轻工技术与工程—食品科学]