响应面法优化短梗霉多糖培养条件  

Optimization of Pullulan Production Conditions by Response Surface Methodology

作　　者：崔玉海[1] 童群义[2] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2008年第3期66-69,共4页Food and Fermentation Industries

基　　金：国家高技术研究发展计期(2007AA102Z362)

摘　　要：采用响应面方法对出芽短梗霉(Aureobasidium pullulans)生产短梗霉多糖的培养条件进行了优化。首先利用Plackett-Burman设计法研究了培养条件对响应值的影响程度,发现(NH_4)_2SO_4和K_2HPO_4的质量浓度对多糖产量的影响显著。然后利用最陡爬坡法逼近最大响应区域.最后在上升最高点处由中心组合试验和响应面分析确定其最优培养条件。并通过实验测得优化培养条件后的多糖产量为24.652 g/L,与预测值24.597 g/L非常接近,且比优化前多糖产量提高了28.4%。Response surface methodology was used to optimize pullulan production conditions for Aureobasidium pullulans. Firstly, the impact of initial conditions on polysaccharides yield was studied by the Plackett-Burman design, revealing that both ammonium sulfate and dipotassium hydrogen phosphate have a substantial influence on polysaccharides yield. Then the greatest response area was estimated by the design of steepest ascent approach. At last, the central compo-site design and response surface analysis were used to estimate optimum conditions. The experimental polysaccharides yield could reach 24. 652g/L under optimized medium, Which agreed with the predicted value of 24. 597g/L and was increased by 28.4 %.

关 键 词：短梗霉多糖 响应面 优化 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]