适合乳果糖生产的β-半乳糖苷酶高产株的筛选及培养基优化  

Screening of high-β-galactosidase-yield strain and optimization of fermentation medium for lactulose production

作　　者：张萃荟[1] 杨瑞金[2] 张文斌[2] 刘芳[2] 费颖[2] 

机构地区：[1]食品科学与技术国家重点实验室(江南大学),江苏无锡214122 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品工业科技》2008年第4期68-70,74,共4页Science and Technology of Food Industry

基　　金：国家高技术研究发展计划(863计划)资助项目(2006AA10Z336)

摘　　要：通过紫外、超声和甲基磺酸乙酯(EMS)诱变乳酸克鲁维酵母(Kluyveromyces lactis),筛选出一株适合乳果糖生产的β-半乳糖苷酶高产菌株K1-16-7,其乳果糖产量达到44.88g/L。通过Plackett-Burman实验设计、中心组合实验设计对其摇瓶培养基进行优化,采用优化配方酶活可以达到147.6U/g。The UV, ultrasonic and ethylmethane sulfonate（EMS） were used for mutating Kluyveromyces lactis. The high-β-galactosidase-yield strain K1-16-7 which was suitable for lactulose production was screened out. The yield of lactulose can reached 44.88g/L. The culture medium was optimizated by Plackett-Burman design and central composite design. After the optimization, the yield of β-galactosidase could reached 147.6U/g.

关 键 词：乳酸克鲁维酵母 诱变育种 Β-半乳糖苷酶 乳果糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]