山药糖蛋白的分离纯化与鉴定  

Study on Isolation, Purification and Identification of Glycoprtein from Chinese Yam

作　　者：丁青芝[1] 马海乐[1] 骆琳[1] 李金忠[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《食品科学》2008年第7期217-220,共4页Food Science

摘　　要：山药粗糖蛋白用DEAE-52纤维素层析纯化,得到了一种中性糖蛋白组分(GLP),用Sephadex G-75进一步进行层析;用薄层层析鉴定了GLP的纯度。用Sephadex G-75柱层析测定了GLP的分子量为89381D;薄层层析法测定了GLP的单糖组成,结果表明,GLP的单糖组成主要有:L-半乳糖、D-葡萄糖、D-甘露糖;对GLP进行了红外光谱扫描,其特征吸收峰再次表明该组分为一种糖蛋白。Glycoprtein from Chinese yam purified by DEAE-cellulose 52. It was successful to obtain one fraction GLP by the step gradient elution. GLP was further purified by Sephadex G-75. TLC was applied to identify the purity of the GLP. The molecular weight of GLP measured by Sephadex G-75 is 89381 D. The experimental result of TLC revealed that the monosaccharide components of GLP are L-galactose, D-glucose and D-mannose. GLP is confirmed by its characteristic absorption peak of infrared spectrum.

关 键 词：山药 糖蛋白 纯化 性质 

分 类 号：TS201.1[轻工技术与工程—食品科学]