一株高效利用木糖的酵母菌的分离及鉴定  

Isolation and identification of one yeast strain with high xylose-utilizing efficiency

作　　者：张凌燕[1] 张梁[1] 王正祥[1] 石贵阳[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室

出　　处：《生物加工过程》2008年第4期56-60,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20706024);国家863计划资助项目(2007AA10Z359)

摘　　要：从256个自然试样中筛选到1株高效转化D-木糖为木糖醇的酵母菌株441-28-1。初始木糖质量浓度为90 g/L的条件下,24 h内的木糖利用效率为3.0 g/(L.h)。通过高效液相分析,菌株441-28-1的主要代谢产物为木糖醇。在初始木糖质量浓度为65 g/L的条件下,摇瓶分批发酵,木糖醇生成速率达1.1 g/(L.h),木糖醇转化率为70%。经过形态、生理生化特征测定,以及ITS序列分析(GenBank的登记号为EU121523),将441-28-1菌株鉴定为热带假丝酵母(Candida tropicalis)。Candida tropicalis(热带假丝酵母)已保存于中国高校工业微生物资源数据平台,保藏编号CICIM Y0092。One yeast strain isolated from 256 natural samples was used for D-xylose. With the initial xylose concentration of 90 g/L, the xylose-utilizing efficiency was 3 g/（L-h） within 24 h. HPLC analysis showed that the main metabolic product was xylitol. With the initial xylose concentration of 65 g/L, the xylitol yield was 0. 7 g/g and the volume productivity was 1.1 g/（L-h）. According to the morphological characteristics, assimilation physiological tests and analysis of ITS nucleotide sequence （GenBank accession number EU121523 ） , the strain was identified as a strain of Candida tropicalis. The strain was submitted to the Culture and Information Centre of Industrial Microorganisms of China Universities and the corresponding serial number was CICIM Y0092.

关 键 词：热带假丝酵母 木糖 木糖醇 乙醇 

分 类 号：Q532.2[生物学—生物化学]