产植酸酶菌株的筛选及其产酶条件研究  

Screening of phytase producing strain and optimization of fermentation conditions

作　　者：杨燕凌[1] 方金铜[1] 黄添孙 

机构地区：[1]福建农林大学生命科学学院,福州350002 [2]武夷山市农业科学研究所,武夷山354300

出　　处：《中国生态农业学报》2008年第4期944-947,共4页Chinese Journal of Eco-Agriculture

基　　金：福建省科技计划重点项目(2007N0094);福建省自然科学基金项目(2007J0243;X0750005);福建省教育厅科技计划项目(JA07059)资助

摘　　要：本研究筛选出1株稳定的产植酸酶菌株,初步鉴定为黑曲霉,命名为Aspergullus niger FZ41。摸索了液体发酵产酶的最佳条件,获得其最适发酵培养基(w/w):可溶性淀粉4%,NH4NO30.08%,KCl0.05%,MgSO.47H2O0.05%,FeSO4.7H2O0.003%,MnSO4.7H2O0.003%,水1L。培养基起始pH值6.0,种龄为36h,接种量为5%,装液量为50/500mL,在30℃、220r·min-1下培养84h,酶活可达219U·mL-1。A phytase-producing strain was isolated and identified as AsperguUus niger FZA1. Liquid fermentation was investigated and the optimal fermentation medium obtained. A 500 mL flask filled with 50 mL inoculums with an initial pH of 6.0 was used in the inoculation. The relative percents of ingredients of fermentation medium were： starch 4% , NH4 NO3 0.08% , KCl 0.05% , MgSO4·7H20 0.05% , FeSO4·7H2O 0.003% , MnSO4·7H2O 0.003%. The age of the inoculums was 36 h, with initial medium pH 6.0 and an inoculation dose of 5%. Extra-cellular phytase production was 219 U·mL^-1 under fermentation conditions of 30℃ at 220 r.min^-1 for 84 h.

关 键 词：植酸酶 菌株筛选 发酵条件 液体 

分 类 号：TQ925[轻工技术与工程—发酵工程]