响应面法优化灰白翅孢壳发酵产胞外多糖的研究  

Study on optimal producing condition of extracellular polysaccharide of Emericellopsis pallida by response surface method

作　　者：苏俊[1] 冯新忠[1] 邝海菊[1] 艾尔肯.热合曼 古丽斯玛依.艾拜都拉 

机构地区：[1]新疆大学生命科学与技术学院资源微生物室,新疆乌鲁木齐830046

出　　处：《食品工业科技》2008年第8期100-102,共3页Science and Technology of Food Industry

基　　金：科技部教学实验用微生物菌种资源子项目(2005DKA21208-9)

摘　　要：采用单次单因子法和响应面法系统考察了灰白翅孢壳（Emericdlopsis pallida）在不同碳源、氮源、辅助碳源条件下产胞外多糖的情况。以单次单因子法得出的变化规律做预备条件，响应面法综合考虑所有影响因素，研究灰白翅孢壳产胞外多糖的情况。结果表明：当蔗糖为59.5g/L，稻草浸汁为20g/L，氯化铵为2g／L时，温度22-26℃，180r／min摇瓶发酵96h时，胞外多糖产率最大，可达1.5g/L。One factor at a time and response surface method were applied for optimal producing extracellular polysaccharide of Emericellopsis pallida among different hydrocarbons, nitrogenous and auxiliaries, The results obtained from one factor at a time were as preparative condition and systematic analysis of response surface method were used to study extracellular polysaccharide. It was showed that the maximum yield of extracellular polysaccharide was 1.5g/L, with saccharose 59.5g/L straw juice 20g/L and NH4Cl 2g/L, fermentation temperature were 22-26℃, time was 96h and rotate speed was 180r/min.

关 键 词：单次单因子法 响应面法 灰白翅孢壳 胞外多糖 

分 类 号：TS201.3[轻工技术与工程—食品科学]