山药皮总甾体皂甙元提取与测定  

Extraction and Determination of Total Steroidal Sapogenins from Chinese Yam Skin

作　　者：洪艳平[1] 尹忠平[1] 上官新晨[1] 蒋艳[1] 杨武英[1] 沈勇根[1] 吴少福[1] 

机构地区：[1]江西农业大学食品科学与工程学院植物资源开发与利用研究室,南昌330045

出　　处：《中国食品学报》2008年第4期46-51,共6页Journal of Chinese Institute Of Food Science and Technology

基　　金：江西省科技厅农业攻关项目(200305001)

摘　　要：目的:从山药皮中提取总甾体皂甙元并测定其含量。方法:采用超声萃取技术提取山药皮中的总甾体皂甙元。以薯蓣皂甙元为对照品,采用分光光度法测定总甾体皂甙元含量。结果:在单因素试验基础上做L9(34)正交试验,确定最佳提取条件为:超声15min,5%硫酸溶液中水解2h,石油醚回流萃取4h,在此条件下,总甾体皂甙元的提取率为1.467%,相对标准偏差(RSD)为0.89%(n=3)。结论:本测定方法简便、快速,稳定性及重现性好。运用超声处理技术从山药皮中提取总甾体皂甙元效果较好。Objective： To extract and determination total steroidal sapogenins in Chinese yam skin. Methods： Total steroidal sapogenins in Chinese yam skin were extracted by ultrasonic extraction technique and determined its content by ultraviolet spectrophotometry using diosgenin as standard sample. Results： The optimal extraction condition was studied through orthogonal tests [（L9（3^4）] on the basis of single factor test. The extraction rate of total steroidal sapogenins was 1.467% in the optimal extraction conditions were as follows： ultrasonic treatment time 15 min, concentration of sulfuric acid 5%, hydrolysis time in the sulfuric acid 2 h and extraction time in petroleum ether 4 h and RSD was 0.89%（n=3）. Conclusion： The determination method was easy, rapid and with good stability and repeatabitity. The results are satisfactory to extract total steroidal sapogenins in Chinese yam skin using ultrasonic technique.

关 键 词：山药皮 总甾体皂甙元 超声提取 测定 

分 类 号：S567.239[农业科学—中草药栽培]