人肝细胞癌4号染色体长臂缺失的细胞遗传学及分子遗传学研究  

Cytogenetic and Molecular Genetic Alterations on Chromosome 4q in Human Hepatocellular Carcinoma

作　　者：张华坤 王辉云[1] 夏建川[1] 

机构地区：[1]华南肿瘤学国家重点实验室

出　　处：《癌症》2008年第9期998-1005,共8页Chinese Journal of Cancer

摘　　要：肝癌的发生是一个多基因、多途径、多阶段的复杂过程,其中染色体的缺失及对应区域的抑癌基因失活是HCC发生发展的重要生物学过程,4q在HCC中经常发生缺失,提示在4q存在肝癌相关的特异性抑癌基因。本文对HCC中有关4q缺失的研究进行了综述,涉及细胞遗传学及分子遗传学水平,包括利用荧光原位杂交、比较基因组技术及限制性片段多态性、微卫星、单核苷酸多态性的杂合性缺失和芯片等技术所做的研究;归纳了4q在不同国家与地区缺失的热点区域,以及与HBV感染、HCC分化程度及肿瘤大小等临床参数的关系;并且列举了4q上与HCC相关的可能的抑癌基因。The carcinogenesis of hepatocellular carcinoma （HCC） is a multi-factor, multi-step, multi-gene and complicated process. Specific chromosome losses and corresponding inactivation of tumor suppressor genes （TSGs） are frequently detected during the development of HCC. A high frequency of loss on chromosome 4q in HCC has been reported, suggesting that the dysfunction of specific TSGs on this chromosome arm is involved in the development and progression of HCC. In this article, we reviewed the studies on chromosomal loss of 4q in HCC patients using cytogenetic and molecular genetic technologies, such as fluorescence in situ hybridization （FISH）, comparative genomic hybridization （CGH） and loss of heterozygosity （LOH） analysis; we also summarized the regions of chromosome 4q with high frequency of loss in HCC patients from different countries, and discussed their relationships with clinical parameters including hepatitis B virus （HBV） infection, tumor differentiation and tumor size, and listed potential TSGs on chromosome 4q in HCC patients.

关 键 词：肝肿瘤 4q 遗传学 比较基因组 杂合性缺失 抑癌基因 

分 类 号：R735.7[医药卫生—肿瘤]