乳链菌肽高产菌株的选育及其生理特性  

Mutation Breeding of High-yield Nisin Producing Strains and Its Physiological Characters

作　　者：焦中高[1] 陈海昌[1] 刘茵[1] 

机构地区：[1]大连轻工业学院食品工程系

出　　处：《大连轻工业学院学报》1997年第4期57-61,共5页Journal of Dalian Institute of Light Industry

摘　　要：以乳酸乳酸球菌７９ＵＶＳ２２为出发菌株，用紫外线、ＤＥＳ、ＬｉＣｌ等多种理化因子进行诱变处理，获得一株乳链菌肽高产突变株ＤＬ２０３，其效价稳定在２０００ＩＵ／ｍｌ以上。对其生理特性的研究表明，ＤＬ２０３菌的生长及发酵最适温度为３０℃，最适ｐＨ值为７．９，通风可以促进菌体的生长，但对乳链菌肽的产生无影响。A mutant strain DL-203 with high yield of Nisin is obtained from Lactococcus Lactis subsp.Lactis 79UV-S22 treated by different physicochemical factors. The Nisin activities of fermentation medium are higher than 2000 IU/ml. The optimum pH value and temperature for Nisin production are 7.9 and 30°C, respectively. Ventilation increases the cell mass greatly, but exerts little influence on the Nisin production.

关 键 词：乳酸乳酸球菌 乳链菌肽 诱变育种 NISIN 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] TQ927