高效液相色谱同时测定食品中多种甜味剂和防腐剂的研究  

Simultaneous determination of sweetenings and preservatives in foods by high-performance liquid chromatography

作　　者：余涛[1] 叶坚[1] 刘昶[1] 

机构地区：[1]广东省肇庆市疾病预防控制中心,广东肇庆526020

出　　处：《中国卫生检验杂志》2008年第10期2014-2016,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：探讨液相色谱同时测定食品中多种甜味剂和防腐剂的方法。方法：采用ODS C18柱,甲醇-乙酸铵作为流动相,针对不同类型样品,采用不同前处理方法,样品处理液最后于液相色谱仪进行分析定量。结果：乙酰磺胺酸钾、苯甲酸、山梨酸、糖精钠、脱氢乙酸、阿斯巴甜在0～0.32 mg/ml浓度范围内,标准曲线线性关系良好,相关系数r分别为0.9999、0.9999、0.9999、0.9999、0.9999、0.9996;乙酰磺胺酸钾的RSD为0.93%～5.8%、苯甲酸的RSD为0.36%～5.6%、山梨酸的RSD为0.67%～6.8%、糖精钠的RSD为1.9%～4.9%、脱氢乙酸的RSD为2.1%～3.3%、阿斯巴甜的RSD为1.3%～6.4%;乙酰磺胺酸钾加标回收率为93.1%～99.6%、苯甲酸为90.5%～98.7%、山梨酸为91.5%～102.5%、糖精钠为91.8%～100.9%、脱氢乙酸为92.3%～99.8%、阿斯巴甜为90.3%～103.5%。结论：本方法简便、快捷,不但可以同时测定食品中多种甜味剂和防腐剂,而且精密度、准确性均满足检测要求,此外对适应食品中多组分同时检测的需求和发展趋势有着积极的作用。Objective：To develop a method for the simultaneous determination of sweetenings and preservatives in foods by using high-performance liquid chromatography.Methods：According to different categories of foods,to choose respective suited preparation methods of sample,to adopt the separated conditions of the ODS C18 column and methanol-aammonium acetatend.Results：The linear ranges of determination forAK,BA,SOR,SA,DHA and APM were 0～0.32 mg/ml（r=0.9999）,0～0.32mg/ml（r=0.9999）,0～0.32mg/ml（r=0.9999）,0～0.32mg/ml（r=0.9999）,0～0.32mg/ml（r=0.9999）,0～0.32mg/ml（r=0.9996）,respectively.The relative standard derivations for AK,BA,SOR,SA,DHA and APM were 0.93%～5.8%,0.36%～5.6%,0.67%～6.8%,1.9%～4.9%,2.1%～3.3%,1.3%～6.4%,respectively.The recoveries of samples for AK,BA,SOR,SA,DHA and APM were 93.1%～99.6%,90.5%～98.7%,91.5%～102.5%,91.8%～100.9%,92.3%～99.8%,90.3%～103.5%,respectively.Conclusion：The method has the advantages of simplicity,repeatability and sessitivity.It can be used for simultaneous analysis of AK,BA,SOR,SA,DHA and APM in foots.

关 键 词：乙酰磺胺酸钾 糖精钠 阿斯巴甜 苯甲酸 山梨酸 脱氢乙酸 高效液相色谱 

分 类 号：O657.72[理学—分析化学]