芸豆种子蛋白组分及其在种子萌发过程中的变化  

Protein Pattern of Kidney Bean Seed and Changes during Kidney Bean Seed Germination

作　　者：党根友[1] 冯佰利[1] 高小丽[1] 高金锋[1] 高冬丽[1] 柴岩[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《华北农学报》2008年第5期85-88,共4页Acta Agriculturae Boreali-Sinica

基　　金："十一五"科技支撑计划(2006BAD02B08);公益性行业(农业)科研专项(nyhyzx07);陕西省科技攻关项目(2006K01-G17-01);西北农林科技大学育种专项;品种后补助资助

摘　　要：以4个具有明显差异的芸豆品种为材料,采用SDS-PAGE方法研究芸豆种子萌发过程中水溶、盐溶和总蛋白组分变化及种子胚和子叶变化过程中蛋白组分变化。试验结果表明,芸豆种子的子叶、胚含有丰富的蛋白亚基,其中,同一品种子叶和胚贮藏蛋白亚基差异明显,子叶、胚的水溶蛋白、盐溶蛋白和总蛋白相差不大、条带丰富;不同品种之间条带存在差异;在发芽过程中,高分子量蛋白亚基的降解速度快于低分子量蛋白亚基,子叶亚基的降解速度明显慢于胚的亚基降解速度;不同品种间子叶、胚条带差异明显,种子活力高的品种(Y05,Y06)降解速度快于种子活力低的品种(Y09,Y1+1),而蛋白含量高的品种(Y1+1,Y05)与蛋白含量低的品种(Y06,Y09)差异不明显。研究结果为芸豆品种鉴定、品种改良、优质栽培及发芽机理提供理论依据。Four kidney bean cultivars with significant difference were used to research the changes of the protein patterns of the storage proteins in the cotyledon and embryo of three protein kinds（water soluble proteins, salt soluble proteins and total proteins）during kidney bean seed germination by SDS-PAGE, the results showed that：There were plentiful storage proteins in subunits in the cotyledon and embryo, thereinto, the significant difference of the storage proteins in subunits was found in the cotyledon and embryo of one cuhivar, there were less diversities and plentiful storage protein patterns among water soluble proteins, salt soluble proteins and total proteins patterns;There were differences in protein patterns among the cultivars. During kidney bean seed germination the storage prote in subunits of macromolecule were mobi lized more quickly than lowermolecule, the storage prote in subunits of the cotyledon were mobilized more slowly than embryo, there were significant differences in protein patterns of the cotyledon and embryo among the cuhivars, the cultivars of the hige seed vigor（Y05, Y06）were mobilized more rapidly than the varieties of the low seed vigor（Y09, Y1 ＋ 1 ）, and there were not diversities between the varieties of the high seed protein content（Y1 ＋ 1 ,Y05） and the varieties of the low seed protein content（Y06, Y09）. The results provided the theoretical basis for the identification of crop varieties, the im provement of crop varieties, quality culture and the mechanism of germination among the kidney beans.

关 键 词：芸豆 贮藏蛋白 子叶和胚 种子发芽 SDS-PAGE 蛋白亚基 

分 类 号：S529.01[农业科学—作物学]