几种多酚化合物抗氧化性的不同化学评价及相关性分析  

Antioxidant Activities of Some Polyphenols Evaluated by Different Chemical Methods and Correlation Analysis

作　　者：张欣[1] 赵新淮[1] 

机构地区：[1]东北农业大学食品学院,黑龙江哈尔滨150030

出　　处：《食品科学》2008年第10期85-89,共5页Food Science

摘　　要：利用α-脱氧核糖法、邻苯三酚自氧化法、DPPH·法、ABTS+·法、铁氰化钾还原法,对茶多酚、没食子酸、槲皮素、山奈素、芹菜素几种多酚化合物及VE(对照)的抗氧化性进行评价,并采用SAS软件对各种评价方法结果进行相关性分析。结果表明,几种多酚化合物均有一定的抗氧化能力,尤其对ABTS+·具有极强的清除能力,其中没食子酸、槲皮素、茶多酚的抗氧化能力远大于山奈素、芹菜素、VE。相关性分析表明,还原能力法分析结果与DPPH·法、ABTS+·法分析结果,具有极显著的正相关性(p<0.01);ABTS+·法分析结果与DPPH·法、α-脱氧核糖法分析结果,O2-·法分析结果与α-脱氧核糖法分析结果,具有显著的正相关性(p<0.05)。The antioxidant activities of some polyphenols, such as tea polyphenol, gallic acid, quercetin, kaempferol, apigenin and vitamin E （as a control）, were evaluated by different chemical methods, including α -deoxyribose assay, pyrogallol autoxidant assay, DPPH radical scavenging assay, ABTS radical scavenging assay and reducing power assay, and SAS software was applied to analyze the correlation between the results obtained by these methods. It was found that all the polyphenols have antioxidant activity, especially higher scavenging ABTS^＋ · activity. Tea polyphenol, gallic acid, quercetin are higher in antioxidant activity than the others. SAS analysis indicated that the result obtained by reducing power assay has extremely significant positive correlation with that obtained by DPPH · assay or ABTS· assay（p 〈 0.01）. Meanwhile, the result obtained by ABTS^＋ · assay has significant positive correlation with that obtained by DPPH · assay or α -deoxyribose assay（p 〈 0.05）.

关 键 词：多酚化合物 抗氧化能力 相关性分析 

分 类 号：Q586[生物学—生物化学]